This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pOPc-Lac-GST-MYCBP2 Lys2458-Asp2553
Parent Plasmid
pOPc-GST with Lac Operon restored
Expressed
GST-MYCBP2 Lys2458-Asp2553
DU Number
DU71107
Genbank
NM_015057.4
Tag
GST
Species
Human
Sequence of Insert
View Sequence
catatgAAACCAAAGTCTGAACCTCAGCCTAATAAGGTTCGAAAATTTGTGGCCAAGGACAGTGCGGGGCTTCGCATCCGTAGCCACCCTTCCCTTCAGAGTGAGCAGATAGGCATAGTGAAAGTCAATGGAACTATCACTTTTATTGATGAGATCCATAATGATGATGGTGTGTGGCTGAGGCTGAATGATGAGACAATAAAGAAGTATGTCCCTAACATGAATGGTTACACTGAAGCCTGGTGCCTCTCTTTTAATCAACATCTTGGCAAGAGTCTTCTGGTCCCTGTTGACTAAggatcc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPHMKPKSEPQPNKVRKFVAKDSAGLRIRSHPSLQSEQIGIVKVNGTITFIDEIHNDDGVWLRLNDETIKKYVPNMNGYTEAWCLSFNQHLGKSLLVPVD*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
The lac operon was restored in a 2 step process:
1. mutagenic insertion of Lac op downstream of T7 promoter: 18575/18576 into pOPc-GST-MYCBP2 Lys2458-Asp2553 71064
2. LacI promoter and ORF amplified from pETDuet with RT18577/18578 were then incorporated via NEB Builder into product of step 1 re-amplified with RT18579/18580