This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Synonyms
6030441H18Rik; Mtmr15
Plasmid
pBabe.puro FLAG.DU mouse FAN1 D984A R985A
Parent Plasmid
pBabeD puro FLAG1
Expressed
FLAG mouse FAN1 D984A R985A
DU Number
DU53022
Genbank
NM_177893.3 GI:257467663 with silent change a414g
Tag
FLAG
Species
Mouse
Sequence of Insert
View Sequence
GGATCCATGCCGTCACAAAGGAAATCACCTGATCAAAAAAGACCCCGTAGAAGCTTATCTACCAGCAAAACTGCAAAAAGCCAGTGTCATTCGATTACTTCGTATTTTAACAGTGCACCACCTGCTAAACTTGCATGTTCAACTTGTCATAAAATGGTGCCCAGGTATGACCTCATTCGGCACCTTGATGAGTCTTGTGCTAACAACGGTGTTGGTGATGACGTTCAAGTAGAGCCTGCTCAGGCTGGATTAATGAGTCCAACTGTGCCTACATCAGATTTACCCAGTGGTCCTTTAGAGAATGTGACACCTCAGAAATTATCACCACCAAAGAGAAGTTTAATTTCTGTCCAATGTGGTTCAAAACTGGGCATACAACAGCAGACCAGTCCCTACTTTAAAGATGCCTTAGTGTCCAAGGATCAAAATGAGCTTCCAAATCAGAGTGTAGAAATTATGCCTTTGGGAAGTCTGACATCGAAATTGTCCAGACGATACTTAAACGCTAAAAAATCACTCGCTAAGAATGAGGGACTAGCCAGTCAGTGCCCACAGACTTCTCCGTCCACACCTGGCACCAGCCTGACTGATAACTGTCCAGAGATGGAAGACAAAGATGAGGTTTTGAACAGTTCCCAAAAGGAAAACATTTATTCATGTGCACCTCTAAAAGAAGAGAATGCTTCAGAACAGAAGGTAAAGAACAATAAAATAACGGGAGATGAAAGCCAGAAAGCTTCCTGCGGGGAGCCAGCCCTCACCCCTGCATCTGCAGAGCATGCTTCCATATTACTGTCCTCAGATTCAACTCTCGTCAGTAACACGAAATCCTCTCCGGGAGACACTCTTGTAAAGCAAGAAAGTGCCAGAAGGGCAGATGTAGGGCTTGCTGAGCCGCTCGAGGTGCGCAGTCACAAAGAAGTACAAATGACTTTCGATGCTGCGGCCAAAACGCTGGTGTCTGGGGAGGCAGAATCAAATGGTCCTACCGATGTTGACATGTCTGACATGACTACGTGGAGTAACAACCAAGAGCTTGTCAGGGAAGCTGGCAGTGTCTTACACTGCCCTCTGGAGCAGGGGTCCAGCTGCGGTGGCCCCAGTGAGACAGCTCAACTGGCACTGAGTCACCCGTACTACCTGCGGAGCTTCCTGGTGGTGCTGCAGGCCCTCCTTGGGAACGAAGAGGACATGAAGCTCTTCGATGAGCAGGAGAAGGCGATTATCACCAGGTTTTACCAGTTGTCAGCTAGTGGTCAAAAGTTGTATGTGAGGCTCTTTCAACGTAAATTAACCTGGATTAAAATGAGTAAACTGGAGTATGAAGAGATTGCCTCCGACTTAACCCCTGTGGTTGAAGAATTGAAGGACTCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGATGTACTTGAACTCCTTTCTGCTCCTGAGCTGAAAGCCCTGGCCAAAACCTTCCACTTGGTGAGTCCCGGTGGGCAGAAGCAGCAGCTGGTAGACGCCTTTCACAAACTGGCCAAACAGCGCTCAGTCTGCACGTGGGGCAAGACTCAGCCTGGAATCCGAGCAGTGATTTTAAAAAGAGCCAAAGACTTGGCTGGCCGGTCGCTTCGAGTCTGTAAAGGCCCTAGGGCTGTGTTTGCCCGAATCTTGCTTCTGTTCTCATTGACTGATTCCATGGAAGACGAAGAAGCTGCTTGTGGGGGTCAAGGTCAGCTGTCTACTGTGCTATTGGTCAATCTGGGCCGAATGGAGTTTCCTCAGTACACCATCTGCCGGAAGACCCAGATCTTCAGGGACAGAGAGGACCTCATCAGATACGCAGCAGCCGCACACATGCTGAGTGACATCTCAGCTGCCATGGCCAGTGGGAACTGGGAGGACGCTAAGGAGCTTGCTCGGAGTGCAAAAAGGGACTGGGAACAACTGAAAAGCCACCCTTCCCTGAGGTACCACGAGGCCTTGCCGCCCTTCCTGCGCTGCTTTACCGTCGGGTGGATCTACACAAGGATTTCCTCTCGGGCTGTTGAAGTCCTAGAGAGGCTTCACATGTACGAGGAAGCTGTCAAGGAACTTGAAAACCTCTTGTCTCAGAAAATCTATTGTCCTGACAGCAGAGGCCGCTGGTGGGATCGCCTGGCTCTTAACCTGCACCAGCACCTGAAGCGTCTGGAAGAGGCAATTAGGTGCATCAGGGAAGGCCTGGCAGACCCACACGTGAGGACAGGACACCGACTTTCTCTTTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGCAGGAAGTACAAGCACCTCTTCAGCCGGCTGCCCGAGGTTGCCGTGGGAGACGTCAAGCATGTGACCATCACGGGCAGGCTGTGCCCACAGCATGGCATGGGCAAGTCTGTGTTTGTCATGGAGAGTGGAGACGGCGCCAACCCCACCACAGTCCTGTGTTCTGTGGAGGAGCTGGCCCTGGGTTATTACAGGCAGAGTGGCTTTGACCAAGGGATTCATGGAGAAGGGTCCACCTTCAGCACCCTGTGTGGCCTCCTGCTGTGGGATATCATCTTCATGGATGGAATACCAGACGTCTTCAGAAATGCCTACCAGGCATCCCCACTGGATTTGCTCACGGACAGCTTCTTCGCAAGCAGGGAGCAGGCCCTGGAAGCCAGGCTGCAGCTGATCCACAGCGCCCCTGCTGAGAGCCTGCGGGCCTGGGTGGGCGAAGCATGGCAGGCCCAGCAAGGCAGAGTGGCCTCTCTGGTCAGCTGGGACCGCTTCACCTCCCTACAGCAAGCTCAGGATCTTGTCTCCTGCCTCGGGGGTCCTGTCCTCAGTGGTGTGTGCAGGCGCCTGGCTGCTGACTTTCGGCACTGCCGAGGGGGCCTCCCAGACTTGGTGGTGTGGAATTCTCAGAGCCACCATTGCAAGCTGGTGGAGGTGAAAGGCCCCAGTGCCGCTCTTTCATGTAAGCAGATGATCTGGCTGTACGAACTCCAAAAGCTGGGGGCTGATGTAGAAGTGTGCCATGTGGTTGCAGTTGGTGCTAAGAGCAAAGGTCTTGGCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMPSQRKSPDQKRPRRSLSTSKTAKSQCHSITSYFNSAPPAKLACSTCHKMVPRYDLIRHLDESCANNGVGDDVQVEPAQAGLMSPTVPT
SDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTP
GTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARR
ADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQAL
LGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHL
VSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQ
YTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEA
VKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRL
CPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQASPLDLLTDSFFASREQALEAR
LQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVWNSQSHHCKLVEVKGPSAALSCK
QMIWLYELQKLGADVEVCHVVAVGAKSKGLG*
SDLPSGPLENVTPQKLSPPKRSLISVQCGSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKKSLAKNEGLASQCPQTSPSTP
GTSLTDNCPEMEDKDEVLNSSQKENIYSCAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTLVSNTKSSPGDTLVKQESARR
ADVGLAEPLEVRSHKEVQMTFDAAAKTLVSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETAQLALSHPYYLRSFLVVLQAL
LGNEEDMKLFDEQEKAIITRFYQLSASGQKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELSDVLELLSAPELKALAKTFHL
VSPGGQKQQLVDAFHKLAKQRSVCTWGKTQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACGGQGQLSTVLLVNLGRMEFPQ
YTICRKTQIFRDREDLIRYAAAAHMLSDISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIYTRISSRAVEVLERLHMYEEA
VKELENLLSQKIYCPDSRGRWWDRLALNLHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKHLFSRLPEVAVGDVKHVTITGRL
CPQHGMGKSVFVMESGDGANPTTVLCSVEELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQASPLDLLTDSFFASREQALEAR
LQLIHSAPAESLRAWVGEAWQAQQGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVWNSQSHHCKLVEVKGPSAALSCK
QMIWLYELQKLGADVEVCHVVAVGAKSKGLG*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet