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TRIM28

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Plasmid
pCMV-HA-2 TRIM 28
Parent Plasmid
pCMV5 HA2
Expressed
HA-TRIM 28
DU Number
DU12452
Genbank
NP_005753.1
Tag
HA
Species
Human
Sequence of Insert
GCGGCCGCGATGGCGGCCTCCGCGGCGGCAGCCTCGGCAGCAGCGGCCTC
GGCCGCCTCTGGCAGCCCGGGCCCGGGCGAGGGCTCCGCTGGCGGCGAAA
AGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGCCTCAGCCGCG
GCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCA
CTGCGGCGTGTGCAGAGAGCGCCTGCGACCCGAGAGGGAGCCCCGCCTGC
TGCCCTGTTTGCACTCGGCCTGTAGTGCCTGCTTAGGGCCCGCGGCCCCC
GCCGCCGCCAACAGCTCGGGGGACGGCGGGGCGGCGGGCGACGGCACCGT
GGTGGACTGTCCCGTGTGCAAGCAACAGTGCTTCTCCAAAGACATCGTGG
AGAATTATTTCATGCGTGATAGTGGCAGCAAGGCTGCCACCGACGCCCAG
GATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCAG
CTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGC
ACCAGCGGGTGAAGTACACCAAGGACCATACTGTGCGCTCTACTGGGCCA
GCCAAGTCTCGGGATGGTGAACGTACTGTCTATTGCAACGTACACAAGCA
TGAACCCCTTGTGCTGTTTTGTGAGAGCTGTGATACTCTCACCTGCCGAG
ACTGCCAGCTCAATGCCCACAAGGACCACCAGTACCAGTTCTTAGAGGAT
GCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGG
GGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAA
TCCGCCAGGTGTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATG
GCCATCCTGCAGATCATGAAGGAGCTGAATAAGCGGGGCCGTGTGCTGGT
CAATGATGCCCAGAAGGTGACTGAGGGGCAGCAGGAGCGCCTGGAGCGGC
AGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCACATTCTGCGC
TTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTC
TAAGAAGTTGATCTACTTCCAGCTGCACCGGGCCCTCAAGATGATTGTGG
ATCCCGTGGAGCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGCC
TGGACCAAGAGTGCCGAGGCCTTTGGCAAGATTGTGGCAGAGCGTCCTGG
CACTAACTCAACAGGCCCTGCACCCATGGCCCCTCCAAGAGCCCCAGGGC
CCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGGAGGTGCAGGAA
GGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCA
TGTGTCAGGTGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCC
TTATGCGCAAGGTGCCACGAGTGAGCCTTGAACGCCTGGACCTGGACCTC
ACAGCTGACAGCCAGCCACCCGTCTTCAAGGTCTTCCCAGGCAGTACCAC
TGAGGACTACAACCTTATTGTTATTGAACGTGGCGCTGCCGCTGCAGCTA
CCGGCCAGCCAGGGACTGCGCCTGCAGGAACCCCTGGTGCCCCACCCCTG
GCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCTGCCATTGGAGC
CCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTC
TTGCGGAGGGTCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGC
AGCACCAGCTCAGGGCTGGAGGTGGTGGCTCCTGAGGGTACCTCAGCCCC
AGGTGGTGGCCCGGGAACCCTGGATGACAGTGCCACCATTTGCCGTGTCT
GCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGAGTTTTGTTTC
CACCTGGACTGTCACCTGCCGGCCCTGCAGGATGTACCAGGGGAGGAGTG
GAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGAGGAGGATGGCA
GCCTCAGCCTGGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCA
CCAGCCAACCAGCGGAAATGTGAGCGTGTACTGCTGGCCCTATTCTGTCA
CGAACCCTGCCGCCCCCTGCATCAGCTGGCTACCGACTCCACCTTCTCCC
TGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCGTGCCCGCCTC
CAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGA
TGTGGGCCGCATGTTCAAGCAATTCAACAAGTTAACTGAGGACAAGGCAG
ACGTGCAGTCCATCATCGGCCTGCAGCGCTTCTTCGAGACGCGCATGAAC
GAGGCCTTCGGTGACACCAAGTTCTCTGCTGTGCTGGTGGAGCCCCCGCC
GATGAGCCTGCCTGGTGCTGGCCTGAGTTCCCAGGAGCTGTCTGGTGGCC
CTGGTGATGGCCCCTGAGCGGCCGC
Amino Acid Sequence
MYPYDVPDYAGIPGIPGSTRAAMAASAAAASAAAASAASGSPGPGEGSAG
GEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRERLRPEREP
RLLPCLHSACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKD
IVENYFMRDSGSKAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCV
EAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLT
CRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVR
SSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERL
ERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKM
IVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRA
PGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEV
SGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAA
AATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVL
MALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATIC
RVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEE
DGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDST
FSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTED
KADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELS
GGPGDGP
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter HA for mammalian expression.

Estimated MW of expressed protein : 90837 Daltons
100042 R 1000 1001 1047 19007
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