TRAF5 | Ubiquitylation Resource

Plasmid

pFBHTb TRAF5

Parent Plasmid

pGEX6P1

Expressed

6His-TRAF5

DU Number

DU3259

Genbank

AAH29600

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GGATCCGCTTATTCAGAAGAGCATAAAGGTATGCCCTGTGGTTTCATCCG
CCAGAATTCCGGCAACTCCATTTCCTTGGACTTTGAGCCCAGTATAGAGT
ACCAGTTTGTGGAGCGGTTGGAAGAGCGCTACAAATGTGCCTTCTGCCAC
TCGGTGCTTCACAACCCCCACCAGACAGGATGTGGGCACCGCTTCTGCCA
GCACTGCATCCTGTCCCTGAGAGAATTAAACACAGTGCCAATCTGCCCTG
TAGATAAAGAGGTCATCAAATCTCAGGAGGTTTTTAAAGACAATTGTTGC
AAAAGAGAAGTCCTCAACTTATATGTATATTGCAGCAATGCTCCTGGATG
TAATGCCAAGGTTATTCTGGGCCGGTACCAGGATCACCTTCAGCAGTGCT
TATTTCAACCTGTGCAGTGTTCTAATGAGAAGTGCCGGGAGCCAGTCCTA
CGGAAAGACCTGAAAGAGCATTTGAGTGCATCCTGTCAGTTTCGAAAGGA
AAAATGCCTTTATTGCAAAAAGGATGTGGTAGTCATCAATCTACAGAATC
ATGAGGAAAACTTGTGTCCTGAATACCCAGTATTTTGTCCCAACAATTGT
GCGAAGATTATTCTAAAAACTGAGGTAGATGAACACCTGGCTGTATGTCC
TGAAGCTGAGCAAGACTGTCCTTTTAAGCACTATGGCTGTGCTGTAACGG
ATAAACGGAGGAACCTGCAGCAACATGAGCATTCAGCCTTACGGGAGCAC
ATGCGTTTGGTTTTAGAAAAGAATGTCCAATTAGAAGAACAGATTTCTGA
CTTACACAAGAGCCTAGAACAGAAAGAAAGTAAAATCCAGCAGCTAGCAG
AAACTATAAAGAAACTTGAAAAGGAGTTCAAGCAGTTTGCACAGTTGTTT
GGCAAAAATGGAAGCTTCCTCCCAAACATCCAGGTTTTTGCCAGTCACAT
TGACAAGTCAGCTTGGCTAGAAGCTCAAGTGCATCAATTATTACAAATGG
TTAACCAGCAACAAAATAAATTTGACCTGAGACCTTTGATGGAAGCAGTT
GATACAGTGAAACAGAAAATTACCCTGCTAGAAAACAATGATCAAAGATT
AGCCGTTTTAGAAGAGGAAACTAACAAACATGATACCCACATTAATATTC
ATAAAGCACAGCTGAGTAAAAATGAAGAGCGATTTAAACTGCTGGAGGGT
ACTTGCTATAATGGAAAGCTCATTTGGAAGGTGACAGATTACAAGATGAA
GAAGAGAGAGGCGGTGGATGGGCACACAGTGTCCATCTTCAGCCAGTCCT
TCTACACCAGCCGCTGTGGCTACCGGCTCTGTGCTAGAGCATACCTGAAT
GGGGATGGGTCAGGGAGGGGGTCACACCTGTCCCTATACTTTGTGGTCAT
GCGAGGAGAGTTTGACTCACTGTTGCAGTGGCCATTCAGGCAGAGGGTGA
CCCTGATGCTTCTGGACCAGAGTGGCAAAAAGAACATTATGGAGACCTTC
AAACCTGACCCCAATAGCAGCAGCTTTAAAAGACCTGATGGGGAGATGAA
CATTGCATCTGGCTGTCCCCGCTTTGTGGCTCATTCTGTTTTGGAGAATG
CCAAGAACGCCTACATTAAAGATGACACTCTGTTCTTGAAAGTGGCCGTG
GACTTAACTGACCTGGAGGATCTCTAGGGATCC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAYSEEHKGMPCGFIRQNSG
NSISLDFEPSIEYQFVERLEERYKCAFCHSVLHNPHQTGCGHRFCQHCIL
SLRELNTVPICPVDKEVIKSQEVFKDNCCKREVLNLYVYCSNAPGCNAKV
ILGRYQDHLQQCLFQPVQCSNEKCREPVLRKDLKEHLSASCQFRKEKCLY
CKKDVVVINLQNHEENLCPEYPVFCPNNCAKIILKTEVDEHLAVCPEAEQ
DCPFKHYGCAVTDKRRNLQQHEHSALREHMRLVLEKNVQLEEQISDLHKS
LEQKESKIQQLAETIKKLEKEFKQFAQLFGKNGSFLPNIQVFASHIDKSA
WLEAQVHQLLQMVNQQQNKFDLRPLMEAVDTVKQKITLLENNDQRLAVLE
EETNKHDTHINIHKAQLSKNEERFKLLEGTCYNGKLIWKVTDYKMKKREA
VDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEF
DSLLQWPFRQRVTLMLLDQSGKKNIMETFKPDPNSSSFKRPDGEMNIASG
CPRFVAHSVLENAKNAYIKDDTLFLKVAVDLTDLEDL*

Vector Type

Baculovirus

Antibiotic

Amp

Comments

N terminal GST for bacterial expresion.
Calc Mol WEight : 91096 Daltons

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