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Synonyms
UBXN8
Full Name
UBXN8
Long Name Textual
UBX domain protein 8
Immuno Sequence
UBXN8 (GST cleaved) [SC 20778]
Mono/Poly clonal
Polyclonal
Aliquot
0.1mg
100041 R 1000 1001 1047 19007
Gel Image
Gel Description
Western Blot with HeLa cell extracts [30 μg/lane] from UBXN8 WT expressing or UBXN8 depleted HeLa cells. Total cell extracts were immunoblotted with purified anti- UBXN8 (R2823) antibody. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-rabbit IgG antibody (1:3000 dilution) followed by enhanced chemiluminescence (ECL, Amersham).
Total cell extracts (0.5 mg) were immunoprecipitated with 60 μl anti- UBXN8 crosslinked to Protein A (PrA) agarose beads (50% slurry). For the antibody crosslinking: 68 μg or 136 μg anti-UBXN8 antibody were crosslinked to 1 ml Protein A agarose beads [50% slurry]. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- rabbit IgG antibody (1:3000 dilution) followed by enhanced chemiluminescence.