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Plasmid
pGEX-TEV-(mu)RNF146 Ala30-Arg183
Parent Plasmid
pGEX-TEV
Expressed
GST-RNF146 (RING-WWE)
DU Number
DU65351
Genbank
NM_001110196.1
Tag
GST
Species
Mouse
Sequence of Insert
View Sequence
GGATCCGCACCTTCTCTGACAGTTCCTGAATGTGCCATTTGTCTACAAACATGTGTTCACCCTGTCAGTCTGCCCTGTAAGCATGTTTTCTGTTATCTGTGTGTAAAGGGTGCTTCATGGCTTGGGAAGCGATGTGCTCTTTGTCGACAAGAGATTCCTGAGGATTTTCTTGACAAGCCAACCTTGTTGTCACCAGAAGAACTTAAGGCTGCAAGCAGAGGAAATGGTGAATATGCGTGGTATTATGAAGGAAGGAATGGGTGGTGGCAGTATGATGAGCGCACTAGTCGGGAGCTAGAAGATGCTTTTTCCAAAGGTAAAAAGAACACGGAAATGTTAATTGCTGGATTTCTGTATGTTGCTGATCTTGAAAACATGGTTCAATATAGGAGAAATGAACATGGACGTCGCAGGAAGATTAAGCGAGATATAATAGATATACCAAAGAAGGGAGTAGCTGGACTTAGGTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSAPSLTVPECAICLQTCVHPVS
LPCKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASR
GNGEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADL
ENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLR*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSAPSLTVPECAICLQTCVHPVS
LPCKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASR
GNGEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADL
ENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLR*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
For more information please look into the biochemical assay in the following paper:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289021/