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Plasmid
pET28a 6HIS TEV HALO-JOSD1
Parent Plasmid
pET28a 6His TEV Halo
Expressed
6HIS TEV HALO-JOSD1
DU Number
DU29561
Genbank
NM_014876.5
Tag
6His
HALO
Species
Human
Sequence of Insert
View Sequence
GGATCCATGAGTTGTGTGCCATGGAAAGGAGACAAGGCCAAATCTGAATC
ATTGGAGCTGCCCCAGGCAGCACCCCCACAAATCTACCATGAGAAACAGC
GCAGGGAGCTTTGTGCCCTCCACGCCCTCAATAACGTCTTCCAGGACAGC
AATGCCTTCACCCGGGATACGCTGCAAGAGATTTTCCAGAGGTTGTCTCC
AAACACCATGGTGACACCTCACAAGAAGAGCATGCTGGGAAATGGCAACT
ACGATGTGAATGTCATTATGGCAGCACTTCAGACCAAAGGCTATGAAGCT
GTTTGGTGGGACAAGCGCAGGGATGTCGGTGTCATTGCCCTCACTAACGT
CATGGGCTTCATCATGAATCTGCCCTCCAGCCTATGCTGGGGTCCACTGA
AACTGCCCCTCAAAAGGCAGCACTGGATCTGTGTTCGAGAGGTGGGAGGG
GCCTACTACAACCTCGACTCCAAACTCAAGATGCCCGAGTGGATTGGAGG
CGAGAGCGAGCTCAGGAAGTTTCTAAAACATCATTTGCGAGGAAAGAACT
GTGAACTCCTGCTGGTGGTACCAGAAGAGGTAGAGGCTCATCAGAGTTGG
AGGACCGATGTGTAAgcggccgc
ATTGGAGCTGCCCCAGGCAGCACCCCCACAAATCTACCATGAGAAACAGC
GCAGGGAGCTTTGTGCCCTCCACGCCCTCAATAACGTCTTCCAGGACAGC
AATGCCTTCACCCGGGATACGCTGCAAGAGATTTTCCAGAGGTTGTCTCC
AAACACCATGGTGACACCTCACAAGAAGAGCATGCTGGGAAATGGCAACT
ACGATGTGAATGTCATTATGGCAGCACTTCAGACCAAAGGCTATGAAGCT
GTTTGGTGGGACAAGCGCAGGGATGTCGGTGTCATTGCCCTCACTAACGT
CATGGGCTTCATCATGAATCTGCCCTCCAGCCTATGCTGGGGTCCACTGA
AACTGCCCCTCAAAAGGCAGCACTGGATCTGTGTTCGAGAGGTGGGAGGG
GCCTACTACAACCTCGACTCCAAACTCAAGATGCCCGAGTGGATTGGAGG
CGAGAGCGAGCTCAGGAAGTTTCTAAAACATCATTTGCGAGGAAAGAACT
GTGAACTCCTGCTGGTGGTACCAGAAGAGGTAGAGGCTCATCAGAGTTGG
AGGACCGATGTGTAAgcggccgc
Amino Acid Sequence
View Sequence
MGSSHHHHHHENLYFQGGMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDG
TPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFD
DHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFI
RPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLT
EVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQS
PVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
GSEIARWLSTLEISGGSMSCVPWKGDKAKSESLELPQAAPPQIYHEKQRR
ELCALHALNNVFQDSNAFTRDTLQEIFQRLSPNTMVTPHKKSMLGNGNYD
VNVIMAALQTKGYEAVWWDKRRDVGVIALTNVMGFIMNLPSSLCWGPLKL
PLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKHHLRGKNCE
LLLVVPEEVEAHQSWRTDV*
TPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFD
DHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFI
RPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLT
EVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQS
PVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
GSEIARWLSTLEISGGSMSCVPWKGDKAKSESLELPQAAPPQIYHEKQRR
ELCALHALNNVFQDSNAFTRDTLQEIFQRLSPNTMVTPHKKSMLGNGNYD
VNVIMAALQTKGYEAVWWDKRRDVGVIALTNVMGFIMNLPSSLCWGPLKL
PLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKHHLRGKNCE
LLLVVPEEVEAHQSWRTDV*
Vector Type
Bacterial
Antibiotic
Kan
Comments
Parental 23918
100042 R 1000 1001 1047 19007
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