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Synonyms
PHA2D
Plasmid
pMK-RQ KLHL3 Cter GFP donor
Parent Plasmid
pMK-RQ
DU Number
DU52069
Genbank
ENSG00000146021
Species
Human
Sequence of Insert
View Sequence
ATAGTGCAGGTTGACTGGTGGCAGAGCTAGGAATGGAATTCAGTCTTTCTTGCTCCCATGAAGGCAGCCTGCTGTACTTTCCGCTTCATTCCAGTGTCTTCTAAAAGTAGAGATGAATCACCCATTGCTTCTCTTACAGTGATTCAAATTTAGAACTGCATAGCTGCCCCTGGGCGCTGAGCATTGTTGGGCTGGGTGCTAGGGCTGGGGATGGGAGTCACCACAGTGAGTCCATGTGATGACACAGGGTGAGGCCACTAGTCAGTGGTGTTTGAGGGACTGTGCTCGTAGGTTCAAGTGTCTTGCCAGGACTTGGCTGTTGGAGTGGGGAACAGCCAGCTGCTAGCAGCCTGAGTCCACTTCAATATTAGGATAAAAATTGCACACATTTCCCTTGGCTGTGACTCAGGATATCTTGGATTCATGAACTCACTGGGACTCTGAGTAATTTTGGAGAAGCTAAGATGATAACTTTCTCCCCCTTTCTTATTAGGTGTTGCaGTGATTCACAAGTCCTTGGGTGGAGGCGGAGGTgtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagTGACCCAAACTCCTACTGCCAGGActTGGAGGAAGGAGCAGGTGCTGCCTGTGACTCTGAACAGCAGGACCTTGGTGACTGGATTCAACTTGCTTGGGAGGGTCTGTGCTGCTGTGAGAACCGCTCTCCTCTGACTTGGCAGACTGGTGTTGTTCATCGCAGTGTGGACACCATTACCCACCCCCGTTCCCCTGAGGTGCTCTGGCCTATGCCCTGAGCAAGGGGGGTCTTGACATCCCCAGGCAGCACCTTTGGGCTTTGTTTTGGTGTTTCTACAGGGACAATACAGACCCTGGAGTGTGTGTGTGTGTGTGTGTGTGTGTAGACCATGGTGTTTCTCTATGTTTCTCTAAGTTGGGGGGTGAGCGTGTGTGACAGTCTACTGGATTTCTTTACTACTGATCCTTTCGCTGTGTTAAAAATCAAGTCACAGAGACCTCTCTTCTGGATTTGTCCCATGGGGACCCTGAGACTACTAAAGCTGCTTTCTTCTGAAGGTCCAGTTGGACAGTCTGGGAATGTCCAG
Vector Type
Other
Antibiotic
Kan
Comments
CRISPR donor plasmid. Designed to KI a c-terminal GFP reporter to KLHL3 when co-transfected with guides 48975 and 48979. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox and may be used in any cell-line.
100042 R 1000 1001 1047 19007
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