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Synonyms
YNL119W; TUC2
Plasmid
pRS413-ADH Pr-3HA-NCS6-Cyc1 ter; ADH Pr-Strep2-FLAG-NCS2-Cyc1 ter
Parent Plasmid
pRS413
Expressed
2XSTREP-FLAG-NCS2
DU Number
DU24731
Genbank
NC_001146.8
Tag
FLAG
StrepII
Species
Saccharomyces cerevisiae
Sequence of Insert
View Sequence
GGGCCCGGTGGAGGCGGTGGAGGCGCCGGGATCGAAGAAATGATGGTAAATGAAATAGGAAATCAAGGAGCATGAAGGCAAAAGACAAATATAAGGGTCGAACGAAAAATAAAGTGAAAAGTGTTGATATGATGTATTTGGCTTTGCGGCGCCGAAAAAACGAGTTTACGCAATTGCACAATCATGCTGACTCTGTGGCGGACCCGCGCTCTTGCCGGCCCGGCGATAACGCTGGGCGTGAGGCTGTGCCCGGCGGAGTTTTTTGCGCCTGCATTTTCCAAGGTTTACCCTGCGCTAAGGGGCGAGATTGGAGAAGCAATAAGAATGCCGGTTGGGGTTGCGATGATGACGACCACGACAACTGGTGTCATTATTTAAGTTGCCGAAAGAACCTGAGTGCATTTGCAACATGAGTATACTAGAAGAATGAGCCAAGACTTGCGAGACGCGAGTTTGCCGGTGGTGCGAACAATAGAGCGACCATGACCTTGAAGGTGAGACGCGCATAACCGCTAGAGTACTTTGAAGAGGAAACAGCAATAGGGTTGCTACCAGTATAAATAGACAGGTACATACAACACTGGAAATGGTTGTCTGTTTGAGTACGCTTTCAATTCATTTGGGTGTGCACTTTATTATGTTACAATATGGAAGGGAACTTTACACTTCTCCTATGCACATATATTAATTAAAGTCCAATGCTAGTAGAGAAGGGGGGTAACACCCCTCCGCGCTCTTTTCCGATTTTTTTCTAAACCGTGGAATATTTCGGATATCCTTTTGTTGTTTCCGGGTGTACAATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTATAATACCTTCGTTGGTCTCCCTAACATGTAGGTGGCGGAGGGGAGATATACAATAGAACAGATACCAGACAAGACATAATGGGCTAAACAAGACTACACCAATTACACTGCCTCATTGATGGTGGTACATAACGAACTAATACTGTAGCCCTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCATTTGCCATCTATTGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTGTCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTCCAAGCTGGCCGCCTAACCGGATCCATGTGGAGCCACCCGCAGTTCGAAAAATGGAGCCACCCGCAGTTCGAAAAAGGCGGAGGCGGTGGAATGGACTACAAGGACGATGACGATAAGGAATTCATGTGGAGCCACCCGCAGTTCGAAAAATGGAGCCACCCGCAGTTCGAAAAAGGCGGAGGCGGTGGAATGGACTACAAGGACGATGACGATAAGGAATTCATGGAGTGCCAGAGGTGCCCTGCATCTGCCAGAAATCCAGCGACAGTTGAGTCTAGAAAGGAAAAGTTTTGCGATGAATGTTTCATCAAGTTTGTTAGTACTAAACAAAGGAAACAAATGATGAAGGATGAATATTTCAGGAACCTATTTAAAGTTATATACCCATTTGAAAAAGAAGGATCCGTCTCTAAGATTCTGCTCCCCTTATCACATTCTGATTCAGGATCTCTGGTTATGTTAGATATTGTGCACGACTTGCTATTGGAACAAACTAAGCAACACAATAATCGTACAGGCTTTACCGTCGATGTACTCACGGTATTCACCGAGGAAAATGTTTCTGTTATCAAAGAACGTATGGAATCCTTGATTAATGAAAAAATGTCACAACTGAATAAAATATCCAACATATTTAATGTCCATTTTATTGATGTTAACGAATTTTTCAACAATGCATCGGAAGTATCGACTTTTATCATTGACAATGAAAATTTTGAGATTTTCAGCAAGTCAAAGTCAGTAGATGATAGCAATATATTAACATTAAAAGAAATATTAGGCAAATACTGTCTCAATAATTCATCCAGGTCTGATTTAATATCGATTATAAAAACTCAATTGATCAAGCATTTTGCATACGAAAATGGTTATAATGCCATAATGTGGGGACATTCCATGACAAAGCTATCTGAAGTAATTATTTCTCTTGTGGTAAAAGGTAAAGGTTCTCAAATAGCGACGTTTCTAGACTCGGAATCATTTGATACTTTGAATAATAAACCATGTAAGTATAAAAACCTATATCCTATGAAAGATCTATTATCCGTCGAGATTGAAAGTTTTTTACAAATCAGAAATCTGGCACAGTTCCTCATTAACGTTGAAGAAACTAATGTGAAGCCTAATTGCTTGATCGCCAGAAAATCACTGCCGTCACTTGGTCAACAAAAATTGGTAAAAAATATGACAATCAACGAAATTACCAACAAATACTTTCAGGATATTCAGAACGATTATTCAAATATAATATCGACAGTTTTAAGAACCGCAGATAAATTGACGCAACCTAAATCAAGTATGGCTAAGCCGTCACAATGTCAAATATGTCAATCTAAAATATACACAAATCCATCCAATTGGTTAAATAGGATCACAGTTACTTCGCCTTATCCTGTGGAGACCACTGAAGAAAAGTATTTATTTAAACAGTGGCAAGATTCAAAATTAGGGCAATCTCACACACATTATGTTGAATTGTTGAATGAAATCAAACAGGGTGCCAGTAATAGCCTCGATGTTGAAGACGGTGATGTCAAATTGTGTTATGGTTGTTTAATCTTATTGAATACCAGTATCAAGGATAAGAATTTGGTTTGGCCAAAAGTCGATACCATGGATATTACGGCAAATGCGACCAATAAAAACAAAGAATTGAGCCAAATATTGGATCAGTTCGAGATTAATTCCGATGGGGAGGAATAAGTCGACCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCTCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTTAATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAAACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTGGTGGAGGCGGTGGAGGCGGGCCC
Amino Acid Sequence
View Sequence
MWSHPQFEKWSHPQFEKGGGGGMDYKDDDDKEFMECQRCPASARNPATVE
SRKEKFCDECFIKFVSTKQRKQMMKDEYFRNLFKVIYPFEKEGSVSKILL
PLSHSDSGSLVMLDIVHDLLLEQTKQHNNRTGFTVDVLTVFTEENVSVIK
ERMESLINEKMSQLNKISNIFNVHFIDVNEFFNNASEVSTFIIDNENFEI
FSKSKSVDDSNILTLKEILGKYCLNNSSRSDLISIIKTQLIKHFAYENGY
NAIMWGHSMTKLSEVIISLVVKGKGSQIATFLDSESFDTLNNKPCKYKNL
YPMKDLLSVEIESFLQIRNLAQFLINVEETNVKPNCLIARKSLPSLGQQK
LVKNMTINEITNKYFQDIQNDYSNIISTVLRTADKLTQPKSSMAKPSQCQ
ICQSKIYTNPSNWLNRITVTSPYPVETTEEKYLFKQWQDSKLGQSHTHYV
ELLNEIKQGASNSLDVEDGDVKLCYGCLILLNTSIKDKNLVWPKVDTMDI
TANATNKNKELSQILDQFEINSDGEE*
SRKEKFCDECFIKFVSTKQRKQMMKDEYFRNLFKVIYPFEKEGSVSKILL
PLSHSDSGSLVMLDIVHDLLLEQTKQHNNRTGFTVDVLTVFTEENVSVIK
ERMESLINEKMSQLNKISNIFNVHFIDVNEFFNNASEVSTFIIDNENFEI
FSKSKSVDDSNILTLKEILGKYCLNNSSRSDLISIIKTQLIKHFAYENGY
NAIMWGHSMTKLSEVIISLVVKGKGSQIATFLDSESFDTLNNKPCKYKNL
YPMKDLLSVEIESFLQIRNLAQFLINVEETNVKPNCLIARKSLPSLGQQK
LVKNMTINEITNKYFQDIQNDYSNIISTVLRTADKLTQPKSSMAKPSQCQ
ICQSKIYTNPSNWLNRITVTSPYPVETTEEKYLFKQWQDSKLGQSHTHYV
ELLNEIKQGASNSLDVEDGDVKLCYGCLILLNTSIKDKNLVWPKVDTMDI
TANATNKNKELSQILDQFEINSDGEE*
Vector Type
Yeast
Antibiotic
Amp
Comments
24563 into 24144 Apa1 to create dual expression vector
100042 R 1000 1001 1047 19007
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