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Synonyms
IsoT-3; ISOT3
Plasmid
pcDNA5 FRT/TO BirA R118G-USP13-HA
Parent Plasmid
pcDNA5 FRT/TO BirA R118G MCS HA
Expressed
BirA R118G-USP13-HA
DU Number
DU49887
Genbank
NM_003940.2 GI:215598687 with silent change t1357c
Tag
HA
BirA
Species
Human
Sequence of Insert
View Sequence
GCTAGCATGCAGCGCCGGGGCGCCCTGTTCGGCATGCCGGGCGGCAGCGGAGGCAGGAAGATGGCTGCAGGAGACATCGGCGAGCTGCTAGTGCCCCACATGCCCACGATCCGCGTGCCCAGGTCCGGCGACAGGGTCTACAAGAACGAGTGCGCCTTCTCCTACGACTCTCCCAATTCTGAAGGTGGACTCTATGTATGCATGAATACATTTTTGGCCTTTGGAAGGGAACATGTTGAAAGACATTTTCGAAAAACTGGACAGAGTGTATACATGCACCTGAAAAGACATGTGCGAGAGAAGGTAAGAGGGGCGTCTGGTGGAGCGTTACCAAAAAGGAGGAATTCCAAGATTTTTTTAGATCTAGATACTGATGACGATTTAAATAGCGACGATTATGAATATGAAGATGAAGCCAAACTTGTTATATTCCCAGATCACTATGAAATAGCACTACCAAATATTGAGGAGTTACCAGCCCTGGTAACAATTGCTTGTGATGCAGTTCTCAGCTCAAAATCTCCATACAGAAAGCAGGACCCAGACACGTGGGAAAATGAATTGCCAGTATCTAAATATGCCAACAACCTCACCCAGCTGGACAATGGAGTCAGGATTCCTCCAAGTGGTTGGAAGTGTGCCAGATGCGACCTGCGAGAAAACCTCTGGTTGAATCTGACTGACGGCTCTGTCCTGTGTGGAAAGTGGTTCTTTGACAGCTCTGGGGGCAACGGGCATGCGCTGGAGCATTACAGAGACATGGGCTACCCACTAGCCGTGAAACTGGGAACCATCACTCCTGACGGGGCAGATGTTTATTCTTTTCAAGAAGAAGAACCTGTTTTGGATCCTCATTTGGCCAAGCACTTAGCGCATTTTGGAATTGATATGCTTCATATGCATGGGACAGAGAATGGGCTCCAGGACAATGACATCAAGCTGAGGGTCAGTGAGTGGGAAGTGATCCAGGAGTCGGGCACGAAACTGAAGCCAATGTATGGTCCTGGCTACACGGGTCTGAAGAACCTGGGCAACAGCTGCTATCTCAGCTCTGTCATGCAGGCCATCTTCAGCATCCCAGAATTCCAGAGAGCGTATGTAGGAAACCTTCCCAGAATATTTGACTACTCGCCTTTAGATCCAACACAAGATTTCAACACACAGATGACTAAGTTAGGACATGGCCTTCTCTCAGGCCAGTATTCAAAGCCTCCGGTGAAATCTGAACTCATTGAACAGGTGATGAAGGAGGAGCACAAGCCACAGCAGAACGGGATCTCTCCGCGCATGTTTAAGGCCTTTGTAAGCAAGAGCCACCCGGAATTCTCCTCTAACAGGCAGCAAGATGCCCAGGAATTCTTCCTGCACCTGGTGAATCTAGTAGAGAGGAACCGCATCGGCTCAGAAAACCCAAGCGATGTTTTTCGTTTTTTGGTGGAAGAACGCATTCAGTGCTGTCAGACCCGGAAAGTCCGCTACACGGAGAGGGTGGATTACCTGATGCAGTTACCTGTGGCCATGGAGGCGGCAACCAACAAGGATGAACTGATCGCTTATGAACTAACGAGAAGGGAAGCAGAAGCAAACAGAAGACCCCTTCCTGAGTTGGTACGTGCCAAGATACCATTTAGTGCCTGCCTTCAGGCCTTCTCTGAACCAGAAAATGTTGATGATTTCTGGAGCAGTGCCCTACAAGCAAAGTCTGCGGGTGTGAAAACATCTCGCTTTGCTTCATTCCCTGAATACTTGGTAGTGCAGATAAAGAAGTTCACTTTTGGTCTTGACTGGGTTCCCAAAAAATTTGATGTTTCTATTGATATGCCAGACCTACTTGATATCAACCATCTCCGAGCCAGGGGGTTACAGCCAGGAGAGGAAGAACTTCCAGACATCAGCCCCCCCATAGTCATTCCTGATGACTCAAAAGATCGCCTGATGAACCAATTGATAGACCCATCAGACATCGATGAGTCATCAGTGATGCAGCTGGCCGAGATGGGTTTCCCGCTGGAAGCATGTCGCAAGGCTGTGTACTTCACTGGAAATATGGGCGCCGAGGTGGCCTTCAACTGGATCATTGTTCACATGGAAGAGCCAGATTTTGCTGAGCCGCTGACCATGCCTGGTTATGGAGGGGCAGCTTCTGCTGGAGCCTCTGTTTTTGGTGCTTCTGGACTGGATAACCAACCTCCAGAGGAAATCGTAGCTATCATCACCTCCATGGGATTTCAGCGAAATCAGGCTATTCAGGCACTACGAGCAACGAATAATAACCTGGAAAGAGCACTGGATTGGATCTTTAGCCACCCTGAGTTTGAAGAAGACAGTGATTTTGTGATTGAGATGGAGAATAATGCCAATGCAAACATTATTTCTGAGGCCAAGCCCGAAGGACCTAGAGTCAAGGATGGATCTGGAACATATGAGCTATTTGCATTCATCAGTCACATGGGAACATCCACAATGAGTGGTCATTACATTTGCCATATCAAAAAGGAAGGAAGATGGGTGATTTACAATGACCACAAAGTTTGTGCCTCAGAAAGGCCCCCTAAAGACCTGGGCTACATGTACTTTTACCGCAGGATACCAAGCGCGGCCGC
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFASMQRRGALFGMPGGSGGRKMAA
GDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEGGLYVCMNTFLA
FGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDLD
TDDDLNSDDYEYEDEAKLVIFPDHYEIALPNIEELPALVTIACDAVLSSK
SPYRKQDPDTWENELPVSKYANNLTQLDNGVRIPPSGWKCARCDLRENLW
LNLTDGSVLCGKWFFDSSGGNGHALEHYRDMGYPLAVKLGTITPDGADVY
SFQEEEPVLDPHLAKHLAHFGIDMLHMHGTENGLQDNDIKLRVSEWEVIQ
ESGTKLKPMYGPGYTGLKNLGNSCYLSSVMQAIFSIPEFQRAYVGNLPRI
FDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQQ
NGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSD
VFRFLVEERIQCCQTRKVRYTERVDYLMQLPVAMEAATNKDELIAYELTR
REAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKT
SRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQP
GEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEAC
RKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGA
SGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFE
EDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMS
GHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPSAAAYPYDV
PDYA*
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFASMQRRGALFGMPGGSGGRKMAA
GDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEGGLYVCMNTFLA
FGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDLD
TDDDLNSDDYEYEDEAKLVIFPDHYEIALPNIEELPALVTIACDAVLSSK
SPYRKQDPDTWENELPVSKYANNLTQLDNGVRIPPSGWKCARCDLRENLW
LNLTDGSVLCGKWFFDSSGGNGHALEHYRDMGYPLAVKLGTITPDGADVY
SFQEEEPVLDPHLAKHLAHFGIDMLHMHGTENGLQDNDIKLRVSEWEVIQ
ESGTKLKPMYGPGYTGLKNLGNSCYLSSVMQAIFSIPEFQRAYVGNLPRI
FDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQQ
NGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSD
VFRFLVEERIQCCQTRKVRYTERVDYLMQLPVAMEAATNKDELIAYELTR
REAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKT
SRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQP
GEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEAC
RKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGA
SGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFE
EDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMS
GHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPSAAAYPYDV
PDYA*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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