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NCS2

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Synonyms
YNL119W; TUC2
Plasmid
pRS413-ADH Pr-3HA-NCS6-Cyc1 ter; ADH Pr-3HA-NCS2-Cyc1 ter
Parent Plasmid
pRS413 pADH 3HA
Expressed
3HA-NCS2
DU Number
DU24532
Genbank
NC_001146.8
Tag
3HA
Species
Saccharomyces cerevisiae
Sequence of Insert
GGGCCCggtggaggcggtggaggcGCCGGGATCGAAGAAATGATGGTAAA
TGAAATAGGAAATCAAGGAGCATGAAGGCAAAAGACAAATATAAGGGTCG
AACGAAAAATAAAGTGAAAAGTGTTGATATGATGTATTTGGCTTTGCGGC
GCCGAAAAAACGAGTTTACGCAATTGCACAATCATGCTGACTCTGTGGCG
GACCCGCGCTCTTGCCGGCCCGGCGATAACGCTGGGCGTGAGGCTGTGCC
CGGCGGAGTTTTTTGCGCCTGCATTTTCCAAGGTTTACCCTGCGCTAAGG
GGCGAGATTGGAGAAGCAATAAGAATGCCGGTTGGGGTTGCGATGATGAC
GACCACGACAACTGGTGTCATTATTTAAGTTGCCGAAAGAACCTGAGTGC
ATTTGCAACATGAGTATACTAGAAGAATGAGCCAAGACTTGCGAGACGCG
AGTTTGCCGGTGGTGCGAACAATAGAGCGACCATGACCTTGAAGGTGAGA
CGCGCATAACCGCTAGAGTACTTTGAAGAGGAAACAGCAATAGGGTTGCT
ACCAGTATAAATAGACAGGTACATACAACACTGGAAATGGTTGTCTGTTT
GAGTACGCTTTCAATTCATTTGGGTGTGCACTTTATTATGTTACAATATG
GAAGGGAACTTTACACTTCTCCTATGCACATATATTAATTAAAGTCCAAT
GCTAGTAGAGAAGGGGGGTAACACCCCTCCGCGCTCTTTTCCGATTTTTT
TCTAAACCGTGGAATATTTCGGATATCCTTTTGTTGTTTCCGGGTGTACA
ATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTA
TAATACCTTCGTTGGTCTCCCTAACATGTAGGTGGCGGAGGGGAGATATA
CAATAGAACAGATACCAGACAAGACATAATGGGCTAAACAAGACTACACC
AATTACACTGCCTCATTGATGGTGGTACATAACGAACTAATACTGTAGCC
CTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCAT
TTGCCATCTATTGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTT
TTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAA
AAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCA
ACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAA
ACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTA
TACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTG
TCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCT
CGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATAT
TTCAAGCTATACCAAGCATACAATCAACTCCAAGCTGGCCGCCTAACCgg
atccATGTACCCATACGATGTGCCAGATTACGCCATGTACCCATACGATG
TGCCAGATTACGCCATGTACCCATACGATGTGCCAGATTACGCCgaattc
ATGGAGTGCCAGAGGTGCCCTGCATCTGCCAGAAATCCAGCGACAGTTGA
GTCTAGAAAGGAAAAGTTTTGCGATGAATGTTTCATCAAGTTTGTTAGTA
CTAAACAAAGGAAACAAATGATGAAGGATGAATATTTCAGGAACCTATTT
AAAGTTATATACCCATTTGAAAAAGAAGGATCCGTCTCTAAGATTCTGCT
CCCCTTATCACATTCTGATTCAGGATCTCTGGTTATGTTAGATATTGTGC
ACGACTTGCTATTGGAACAAACTAAGCAACACAATAATCGTACAGGCTTT
ACCGTCGATGTACTCACGGTATTCACCGAGGAAAATGTTTCTGTTATCAA
AGAACGTATGGAATCCTTGATTAATGAAAAAATGTCACAACTGAATAAAA
TATCCAACATATTTAATGTCCATTTTATTGATGTTAACGAATTTTTCAAC
AATGCATCGGAAGTATCGACTTTTATCATTGACAATGAAAATTTTGAGAT
TTTCAGCAAGTCAAAGTCAGTAGATGATAGCAATATATTAACATTAAAAG
AAATATTAGGCAAATACTGTCTCAATAATTCATCCAGGTCTGATTTAATA
TCGATTATAAAAACTCAATTGATCAAGCATTTTGCATACGAAAATGGTTA
TAATGCCATAATGTGGGGACATTCCATGACAAAGCTATCTGAAGTAATTA
TTTCTCTTGTGGTAAAAGGTAAAGGTTCTCAAATAGCGACGTTTCTAGAC
TCGGAATCATTTGATACTTTGAATAATAAACCATGTAAGTATAAAAACCT
ATATCCTATGAAAGATCTATTATCCGTCGAGATTGAAAGTTTTTTACAAA
TCAGAAATCTGGCACAGTTCCTCATTAACGTTGAAGAAACTAATGTGAAG
CCTAATTGCTTGATCGCCAGAAAATCACTGCCGTCACTTGGTCAACAAAA
ATTGGTAAAAAATATGACAATCAACGAAATTACCAACAAATACTTTCAGG
ATATTCAGAACGATTATTCAAATATAATATCGACAGTTTTAAGAACCGCA
GATAAATTGACGCAACCTAAATCAAGTATGGCTAAGCCGTCACAATGTCA
AATATGTCAATCTAAAATATACACAAATCCATCCAATTGGTTAAATAGGA
TCACAGTTACTTCGCCTTATCCTGTGGAGACCACTGAAGAAAAGTATTTA
TTTAAACAGTGGCAAGATTCAAAATTAGGGCAATCTCACACACATTATGT
TGAATTGTTGAATGAAATCAAACAGGGTGCCAGTAATAGCCTCGATGTTG
AAGACGGTGATGTCAAATTGTGTTATGGTTGTTTAATCTTATTGAATACC
AGTATCAAGGATAAGAATTTGGTTTGGCCAAAAGTCGATACCATGGATAT
TACGGCAAATGCGACCAATAAAAACAAAGAATTGAGCCAAATATTGGATC
AGTTCGAGATTAATTCCGATGGGGAGGAATAAgtcgacCATGTAATTAGT
TATGTCACGCTTACATTCACGCCCTCCTCCCACATCCGCTCTAACCGAAA
AGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTTAA
TAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTT
TTTCTGTACAAACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCT
TGAGAAGGTTTTGGGACGCTCGAAGGCTTTggtggaggcggtggaggcGG
GCCC
Amino Acid Sequence
MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFMECQRCPASARNPATVES
RKEKFCDECFIKFVSTKQRKQMMKDEYFRNLFKVIYPFEKEGSVSKILLP
LSHSDSGSLVMLDIVHDLLLEQTKQHNNRTGFTVDVLTVFTEENVSVIKE
RMESLINEKMSQLNKISNIFNVHFIDVNEFFNNASEVSTFIIDNENFEIF
SKSKSVDDSNILTLKEILGKYCLNNSSRSDLISIIKTQLIKHFAYENGYN
AIMWGHSMTKLSEVIISLVVKGKGSQIATFLDSESFDTLNNKPCKYKNLY
PMKDLLSVEIESFLQIRNLAQFLINVEETNVKPNCLIARKSLPSLGQQKL
VKNMTINEITNKYFQDIQNDYSNIISTVLRTADKLTQPKSSMAKPSQCQI
CQSKIYTNPSNWLNRITVTSPYPVETTEEKYLFKQWQDSKLGQSHTHYVE
LLNEIKQGASNSLDVEDGDVKLCYGCLILLNTSIKDKNLVWPKVDTMDIT
ANATNKNKELSQILDQFEINSDGEE*
Vector Type
Other
Antibiotic
Amp
Comments
24515 into 24144 Apa1 to create dual expression vector
100042 R 1000 1001 1047 19007
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