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TRIM49

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Synonyms
RNF18, TRIM49L2
Plasmid
pMK-RQ TRIM49 Nter PURO donor
Parent Plasmid
pMK-RQ
Expressed
Puro (following recombination)
DU Number
DU48233
Genbank
ENSG00000168930
Species
Human
Sequence of Insert
CCATGGTGGTTTGCTGCCCGGCTTTTGTGACTCTCTATAAAAATATGTCATAGAACCATCTTCTCATATTTCTTTGGCCATCTTCTCATATTTCTTTGGCCCGGGTAGTTTTTTCCCTCCCACTCTTTCACAGTCATTCATCAGTTATTGAAAGAAGAATAGGGACAACTATGATTTATTCTGACCACTTAGAATCAAAACCCAATTTCCAGATCTATGTTCAGAAAATGGGTAGTTGAATAGGTTTGTATTATGCTACAGACAGGAACTAGGAGTAGAAGGGACTGTGAGTCCTCTGGTTAGTAATATCTGTTAGAAAGCCAGGAGACTCCCTCTAAGTGTAAATTTTTAAGTTTGTAACAGAAGAAATAGTTTGTTGTACTTTAATGAACACTGTCAAGAGAAGAAAATATAACTATCACATATCTCCACATGTTTAGAAGGTTTTCATCAACCCAGGCCCCAAAATGACATGTTTCTCTTTGTTCCTCAGAAACATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGGTAAGTGATGGCTCTGAAGATCGATTTCTGTAAAGGAAGCATAAAATTCCTGTGGGTCTATTTTCTTGGAGATTGGATAAAGCCAACTCTGAGTCCCTTTAAGCAGCTCTGTTTGGGCTTTCTTAGCTTCCAACCTCTGGGCTTTGACAAACATGAAGGGAAACAAAGGAAATGCCATTTACTAGGGGCTTATTTGTCTCTCATTCTGGGCCCCCTCCCTATGTCATGGTCTGCACTGGTGCTTCAATTTATGGCTCTTTTGCAGGAGAAGCTTTTACAGAAAATGCAGTCTTTGTGGGAAAAAGCTTGTGAAAATCACAGAAACCTGAATGTGGAAACCACCAGAACCAGATGCTGGAAGGTTAGTCCTGTACTACTCTACCTTCTCCAGGAACTTATGGTGGGCAAATGGGTGACTCTTAAAATAGGAACTTGATATCAAACTGTAATGTTTCTGGGATACAGTAAAAAAAAAAAGGCGAGAGAAAACACTGAGAAAA
Amino Acid Sequence
MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQ
ELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSG
SRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEA
AERAGVPAFLETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*
Vector Type
Other
Antibiotic
Kan
Comments
CRISPR donor designed to knockout TRIM49 by inserting Puro to the N-terminus (exon 3) via homologous recombination. Consists of a puromycin-polyA cassette flanked on either side by 500 bp of TRIM49 homology and is to be co-transfected with 48096 into a Cas9 expressing cell-line.
Sequence of insert shows the donor sequence whilst sequence of cassette and amino acid sequence depict the expected expression product from the resultant transcript.
100042 R 1000 1001 1047 19007
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