FANCI | Ubiquitylation Resource

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Synonyms

Fanconi anemia, complementation group I, KIAA1794

Plasmid

pCMV-Myc1-FANCI K898E K900E K980E

Parent Plasmid

pCMV5 Myc1

Expressed

Myc-FANCI K898E K900E K980E

DU Number

DU24046

Genbank

NM_001113378.1.

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGACCAGAAGATTTTATCTCTAGCAGCAGAAAAAACAGCAGA
CAAACTGCAAGAATTTCTTCAAACCCTGAGAGAAGGTGATTTGACTAATC
TCCTTCAGAATCAAGCAGTGAAAGGAAAAGTTGCTGGAGCACTCCTGAGA
GCCATCTTCAAAGGTTCCCCCTGCTCTGAGGAAGCTGGAACACTTAGGAG
ACGTAAGATATACACTTGTTGTATCCAGTTGGTGGAATCGGGGGATTTGC
AGAAAGAAATAGCGTCTGAGATCATAGGATTACTGATGCTGGAGGCTCAC
CATTTTCCAGGACCATTATTGGTTGAATTAGCCAATGAGTTTATTAGTGC
TGTCAGAGAAGGCAGCCTAGTGAATGGAAAATCTTTGGAGTTACTACCTA
TCATTCTCACTGCCCTGGCTACGAAAAAGGAAAATCTGGCTTATGGAAAA
GGTGTACTGAGTGGGGAAGAATGTAAGAAACAGTTGATTAACACCCTGTG
TTCTGGCAGGTGGGATCAGCAATATGTAATCCAACTCACCTCCATGTTCA
AGGATGTCCCTCTGACTGCAGAAGAGGTGGAATTTGTGGTGGAAAAAGCA
TTGAGCATGTTCTCCAAGATGAATCTTCAAGAAATACCACCTTTGGTCTA
TCAGCTTCTGGTTCTCTCCTCCAAGGGAAGCAGAAAGAGTGTTTTGGAAG
GAATCATAGCCTTCTTCAGTGCACTAGATAAGCAGCACAATGAGGAACAG
AGTGGTGACGAGCTATTGGATGTTGTCACTGTGCCATCAGGTGAACTTCG
TCATGTGGAAGGCACCATTATTCTACACATTGTGTTTGCCATCAAATTGG
ACTATGAACTAGGCAGAGAACTCGTGAAACACTTAAAGGTAGGACAGCAA
GGAGATTCCAATAATAACTTAAGTCCCTTCAGCATTGCTCTTCTTCTGTC
TGTAACAAGAATACAAAGATTTCAGGACCAGGTGCTTGATCTTTTAAAGA
CTTCGGTTGTAAAGAGCTTTAAGGATCTTCAACTCCTCCAAGGCTCAAAA
TTTCTTCAGAATCTAGTTCCTCATAGATCTTATGTTTCAACCATGATCTT
GGAAGTAGTGAAGAATAGCGTTCATAGCTGGGACCATGTTACTCAGGGCC
TCGTAGAACTTGGTTTCATTTTGATGGATTCATATGGGCCAAAGAAGGTT
CTTGATGGAAAAACTATTGAAACCAGCCCAAGTCTTTCTAGAATGCCAAA
CCAGCATGCATGTAAGCTCGGAGCTAATATCCTGTTGGAAACTTTTAAGA
TCCATGAGATGATCAGACAAGAAATTTTGGAGCAGGTCCTCAACAGGGTT
GTTACCAGAGCATCTTCTCCCATCAGTCATTTCTTAGACCTGCTTTCAAA
TATCGTCATGTATGCACCCTTAGTTCTTCAAAGTTGTTCTTCTAAAGTCA
CAGAAGCTTTTGACTATTTGTCCTTTCTGCCCCTTCAGACTGTACAAAGG
CTGCTTAAGGCAGTGCAGCCCCTTCTCAAAGTCAGCATGTCAATGAGAGA
CTGCTTGATACTTGTCCTTCGGAAAGCTATGTTTGCCAACCAGCTTGATG
CCCGAAAATCTGCAGTTGCTGGGTTTTTGCTGCTCCTGAAGAACTTTAAA
GTTTTAGGCAGCCTGTCATCCTCTCAGTGCAGTCAGTCTCTCAGTGTCAG
TCAGGTTCATGTGGATGTTCACAGCCATTACAATTCTGTCGCCAATGAAA
CTTTTTGCCTTGAGATCATGGATAGTTTGAGGAGATGCTTAAGCCAGCAA
GCTGATGTTCGACTCATGCTTTATGAGGGGTTTTATGATGTTCTTCGAAG
GAACTCTCAGCTGGCTAATTCAGTCATGCAAACTCTGCTCTCACAGTTAA
AACAGTTCTATGAGCCAAAACCTGATCTGCTGCCTCCTCTGAAATTAGAA
GCTTGTATTCTGACCCAAGGAGATAAGATCTCTCTACAAGAACCACTGGA
TTATCTGCTGTGTTGTATTCAGCATTGTTTGGCCTGGTATAAGAATACAG
TCATACCCTTACAGCAGGGAGAGGAGGAAGAGGAGGAGGAAGAGGCATTC
TACGAAGACCTAGATGATATATTGGAGTCCATTACTAATAGAATGATTAA
GAGTGAGCTGGAAGACTTTGAACTGGATAAATCAGCAGATTTTTCTCAGA
GCACCAGTATTGGCATAAAAAATAATATCTGTGCTTTTCTTGTGATGGGA
GTTTGTGAGGTTTTAATAGAATACAATTTCTCCATAAGTAGTTTCAGTAA
GAATAGGTTTGAGGACATTCTGAGCTTATTTATGTGTTACAAAAAACTCT
CTGACATTCTTAATGAAAAAGCGGGTAAAGCCAAAACTAAAATGGCCAAC
AAGACAAGTGATAGTCTTTTGTCCATGAAATTTGTGTCCAGTCTTCTCAC
TGCTCTTTTCAGGGATAGTATCCAAAGCCACCAAGAAAGCCTTTCTGTTC
TCAGGTCCAGCAATGAGTTTATGCGCTATGCAGTGAATGTAGCTCTGCAG
AAAGTACAGCAGCTAAAGGAAACAGGGCATGTGAGTGGCCCTGATGGCCA
AAACCCAGAAAAGATCTTTCAGAACCTCTGTGACATAACTCGAGTCTTGC
TATGGAGATACACTTCAATTCCTACTTCAGTGGAAGAGTCGGGAAAGGAA
GAGGAAGGAAAGAGCATCTCACTGCTGTGCTTGGAGGGTTTACAGAAAAT
ATTCAGTGCTGTGCAACAGTTCTATCAGCCCAAGATTCAGCAGTTTCTCA
GAGCTCTGGATGTCACAGATAAGGAAGGAGAAGAGAGAGAAGATGCAGAT
GTCAGTGTCACTCAGAGAACAGCATTCCAGATCCGGCAATTTCAGAGGTC
CTTGTTGAATTTACTTAGCAGTCAAGAGGAAGATTTTAATAGCGAAGAAG
CCCTCCTGCTAGTCACGGTTCTTACCAGTTTGTCCAAGTTACTGGAGCCC
TCCTCTCCTCAGTTTGTGCAGATGTTATCCTGGACATCAAAGATTTGCAA
GGAAAACAGCCGGGAGGATGCCTTGTTTTGCAAGAGCTTGATGAACTTGC
TCTTCAGCCTGCATGTTTCGTATAAGAGTCCTGTCATTCTGCTGCGTGAC
TTGTCCCAGGATATCCACGGGCATCTGGGAGATATAGACCAGGATGTAGA
GGTGGAGAAAACAAACCACTTTGCAATAGTGAATTTGAGAACGGCTGCCC
CCACTGTCTGTTTACTTGTTCTGAGTCAGGCCGAGAAGGTTCTAGAAGAA
GTGGACTGGCTAATCACCAAGCTTAAGGGACAAGTGAGCCAAGAAACCTT
ATCAGAAGAGGCCTCTTCTCAGGCAACCCTACCAAATCAGCCTGTTGAGA
AAGCTATCATCATGCAACTGGGAACTCTGCTTACATTTTTCCACGAGCTG
GTGCAGACAGCTCTGCCATCAGGCAGCTGTGTGGACACCTTGTTAAAGGA
CTTGTGCAAAATGTACACCACACTTACAGCCCTTGTCAGATATTATCTCC
AGGTGTGTCAGAGCTCCGGAGGAATTCCAAAAAATATGGAAAAGCTGGTG
AAGCTGTCTGGTTCTCATCTGACCCCCCTGTGTTATTCTTTCATTTCTTA
CGTACAGAATAAGAGTAAGAGCCTGAACTATACGGGAGAGAAAAAGGAGA
AACCTGCTGCCGTTGCCACAGCCATGGCCAGAGTTCTTCGGGAAACCAAG
CCAATCCCTAACCTCATCTTTGCCATAGAACAGTATGAAAAATTTCTCAT
CCACCTTTCTAAGAAGTCCAAGGTGAACCTGATGCAGCACATGAAGCTCA
GCACCTCACGAGACTTCAAGATCAAAGGAAACATCCTAGACATGGTTCTT
CGAGAGGATGGTGAAGATGAAAATGAAGAGGGCACTGCATCAGAGCATGG
GGGACAGAACAAAGAACCAGCCAAGAAGAAAAGGAAAAAATAAGCGGCCG
C

Amino Acid Sequence

View Sequence

MMEQKLISEEDLGSMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQ
AVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIA
SEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTA
LATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPL
TAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAF
FSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELG
RELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVK
SFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELG
FILMDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMI
RQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFD
YLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSA
VAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLE
IMDSLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYE
PKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQ
QGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIG
IKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILN
EKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSN
EFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYT
SIPTSVEESGKEEEGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDV
TDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSEEALLLV
TVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLH
VSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCL
LVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIM
QLGTLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQS
SGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAV
ATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSRD
FKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK*

Vector Type

Mammalian

Antibiotic

Amp

Comments

All FANCI plasmids MUST be grown at 30 degrees C or less to prevent recombination.

100042 R 1000 1001 1047 19007

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