FANCI | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-FLAG FANCI R1285Q

Parent Plasmid

pcDNA5 FRT/TO FLAG

Expressed

FLAG-FANCI R1285Q

DU Number

DU24038

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGACCAGAAGATTTTATCTCTAGCAGCAGAAAAAACAGCAGA
CAAACTGCAAGAATTTCTTCAAACCCTGAGAGAAGGTGATTTGACTAATC
TCCTTCAGAATCAAGCAGTGAAAGGAAAAGTTGCTGGAGCACTCCTGAGA
GCCATCTTCAAAGGTTCCCCCTGCTCTGAGGAAGCTGGAACACTTAGGAG
ACGTAAGATATACACTTGTTGTATCCAGTTGGTGGAATCGGGGGATTTGC
AGAAAGAAATAGCGTCTGAGATCATAGGATTACTGATGCTGGAGGCTCAC
CATTTTCCAGGACCATTATTGGTTGAATTAGCCAATGAGTTTATTAGTGC
TGTCAGAGAAGGCAGCCTAGTGAATGGAAAATCTTTGGAGTTACTACCTA
TCATTCTCACTGCCCTGGCTACGAAAAAGGAAAATCTGGCTTATGGAAAA
GGTGTACTGAGTGGGGAAGAATGTAAGAAACAGTTGATTAACACCCTGTG
TTCTGGCAGGTGGGATCAGCAATATGTAATCCAACTCACCTCCATGTTCA
AGGATGTCCCTCTGACTGCAGAAGAGGTGGAATTTGTGGTGGAAAAAGCA
TTGAGCATGTTCTCCAAGATGAATCTTCAAGAAATACCACCTTTGGTCTA
TCAGCTTCTGGTTCTCTCCTCCAAGGGAAGCAGAAAGAGTGTTTTGGAAG
GAATCATAGCCTTCTTCAGTGCACTAGATAAGCAGCACAATGAGGAACAG
AGTGGTGACGAGCTATTGGATGTTGTCACTGTGCCATCAGGTGAACTTCG
TCATGTGGAAGGCACCATTATTCTACACATTGTGTTTGCCATCAAATTGG
ACTATGAACTAGGCAGAGAACTCGTGAAACACTTAAAGGTAGGACAGCAA
GGAGATTCCAATAATAACTTAAGTCCCTTCAGCATTGCTCTTCTTCTGTC
TGTAACAAGAATACAAAGATTTCAGGACCAGGTGCTTGATCTTTTAAAGA
CTTCGGTTGTAAAGAGCTTTAAGGATCTTCAACTCCTCCAAGGCTCAAAA
TTTCTTCAGAATCTAGTTCCTCATAGATCTTATGTTTCAACCATGATCTT
GGAAGTAGTGAAGAATAGCGTTCATAGCTGGGACCATGTTACTCAGGGCC
TCGTAGAACTTGGTTTCATTTTGATGGATTCATATGGGCCAAAGAAGGTT
CTTGATGGAAAAACTATTGAAACCAGCCCAAGTCTTTCTAGAATGCCAAA
CCAGCATGCATGTAAGCTCGGAGCTAATATCCTGTTGGAAACTTTTAAGA
TCCATGAGATGATCAGACAAGAAATTTTGGAGCAGGTCCTCAACAGGGTT
GTTACCAGAGCATCTTCTCCCATCAGTCATTTCTTAGACCTGCTTTCAAA
TATCGTCATGTATGCACCCTTAGTTCTTCAAAGTTGTTCTTCTAAAGTCA
CAGAAGCTTTTGACTATTTGTCCTTTCTGCCCCTTCAGACTGTACAAAGG
CTGCTTAAGGCAGTGCAGCCCCTTCTCAAAGTCAGCATGTCAATGAGAGA
CTGCTTGATACTTGTCCTTCGGAAAGCTATGTTTGCCAACCAGCTTGATG
CCCGAAAATCTGCAGTTGCTGGGTTTTTGCTGCTCCTGAAGAACTTTAAA
GTTTTAGGCAGCCTGTCATCCTCTCAGTGCAGTCAGTCTCTCAGTGTCAG
TCAGGTTCATGTGGATGTTCACAGCCATTACAATTCTGTCGCCAATGAAA
CTTTTTGCCTTGAGATCATGGATAGTTTGAGGAGATGCTTAAGCCAGCAA
GCTGATGTTCGACTCATGCTTTATGAGGGGTTTTATGATGTTCTTCGAAG
GAACTCTCAGCTGGCTAATTCAGTCATGCAAACTCTGCTCTCACAGTTAA
AACAGTTCTATGAGCCAAAACCTGATCTGCTGCCTCCTCTGAAATTAGAA
GCTTGTATTCTGACCCAAGGAGATAAGATCTCTCTACAAGAACCACTGGA
TTATCTGCTGTGTTGTATTCAGCATTGTTTGGCCTGGTATAAGAATACAG
TCATACCCTTACAGCAGGGAGAGGAGGAAGAGGAGGAGGAAGAGGCATTC
TACGAAGACCTAGATGATATATTGGAGTCCATTACTAATAGAATGATTAA
GAGTGAGCTGGAAGACTTTGAACTGGATAAATCAGCAGATTTTTCTCAGA
GCACCAGTATTGGCATAAAAAATAATATCTGTGCTTTTCTTGTGATGGGA
GTTTGTGAGGTTTTAATAGAATACAATTTCTCCATAAGTAGTTTCAGTAA
GAATAGGTTTGAGGACATTCTGAGCTTATTTATGTGTTACAAAAAACTCT
CTGACATTCTTAATGAAAAAGCGGGTAAAGCCAAAACTAAAATGGCCAAC
AAGACAAGTGATAGTCTTTTGTCCATGAAATTTGTGTCCAGTCTTCTCAC
TGCTCTTTTCAGGGATAGTATCCAAAGCCACCAAGAAAGCCTTTCTGTTC
TCAGGTCCAGCAATGAGTTTATGCGCTATGCAGTGAATGTAGCTCTGCAG
AAAGTACAGCAGCTAAAGGAAACAGGGCATGTGAGTGGCCCTGATGGCCA
AAACCCAGAAAAGATCTTTCAGAACCTCTGTGACATAACTCGAGTCTTGC
TATGGAGATACACTTCAATTCCTACTTCAGTGGAAGAGTCGGGAAAGAAA
GAGAAAGGAAAGAGCATCTCACTGCTGTGCTTGGAGGGTTTACAGAAAAT
ATTCAGTGCTGTGCAACAGTTCTATCAGCCCAAGATTCAGCAGTTTCTCA
GAGCTCTGGATGTCACAGATAAGGAAGGAGAAGAGAGAGAAGATGCAGAT
GTCAGTGTCACTCAGAGAACAGCATTCCAGATCCGGCAATTTCAGAGGTC
CTTGTTGAATTTACTTAGCAGTCAAGAGGAAGATTTTAATAGCAAAGAAG
CCCTCCTGCTAGTCACGGTTCTTACCAGTTTGTCCAAGTTACTGGAGCCC
TCCTCTCCTCAGTTTGTGCAGATGTTATCCTGGACATCAAAGATTTGCAA
GGAAAACAGCCGGGAGGATGCCTTGTTTTGCAAGAGCTTGATGAACTTGC
TCTTCAGCCTGCATGTTTCGTATAAGAGTCCTGTCATTCTGCTGCGTGAC
TTGTCCCAGGATATCCACGGGCATCTGGGAGATATAGACCAGGATGTAGA
GGTGGAGAAAACAAACCACTTTGCAATAGTGAATTTGAGAACGGCTGCCC
CCACTGTCTGTTTACTTGTTCTGAGTCAGGCCGAGAAGGTTCTAGAAGAA
GTGGACTGGCTAATCACCAAGCTTAAGGGACAAGTGAGCCAAGAAACCTT
ATCAGAAGAGGCCTCTTCTCAGGCAACCCTACCAAATCAGCCTGTTGAGA
AAGCTATCATCATGCAACTGGGAACTCTGCTTACATTTTTCCACGAGCTG
GTGCAGACAGCTCTGCCATCAGGCAGCTGTGTGGACACCTTGTTAAAGGA
CTTGTGCAAAATGTACACCACACTTACAGCCCTTGTCAGATATTATCTCC
AGGTGTGTCAGAGCTCCGGAGGAATTCCAAAAAATATGGAAAAGCTGGTG
AAGCTGTCTGGTTCTCATCTGACCCCCCTGTGTTATTCTTTCATTTCTTA
CGTACAGAATAAGAGTAAGAGCCTGAACTATACGGGAGAGAAAAAGGAGA
AACCTGCTGCCGTTGCCACAGCCATGGCCAGAGTTCTTCGGGAAACCAAG
CCAATCCCTAACCTCATCTTTGCCATAGAACAGTATGAAAAATTTCTCAT
CCACCTTTCTAAGAAGTCCAAGGTGAACCTGATGCAGCACATGAAGCTCA
GCACCTCACaAGACTTCAAGATCAAAGGAAACATCCTAGACATGGTTCTT
CGAGAGGATGGTGAAGATGAAAATGAAGAGGGCACTGCATCAGAGCATGG
GGGACAGAACAAAGAACCAGCCAAGAAGAAAAGGAAAAAATAAGCGGCCG
C

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVK
GKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIASEI
IGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALAT
KKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAE
EVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSA
LDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGREL
VKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFK
DLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFIL
MDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQE
ILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLS
FLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAG
FLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLEIMD
SLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKP
DLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQGE
EEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKN
NICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKA
GKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFM
RYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIP
TSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDK
EGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALLLVTVL
TSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHVSY
KSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVL
SQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLG
TLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGG
IPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATA
MARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSQDFKI
KGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK*AA

Vector Type

Mammalian

Antibiotic

Amp

Comments

All FANCI plasmids MUST be grown at 30 degrees C or less to prevent recombination.

100042 R 1000 1001 1047 19007

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