UBE2D2

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Plasmid
pET28-UBE2D2
Parent Plasmid
pET28a original
Expressed
6His-UBE2D2
DU Number
DU20184
Genbank
NM_003339
Tag
6His
Species
Human
Sequence of Insert
GGATCCGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGG
GACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCA
TTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAG
TATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCT
AAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGG
CAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTA
TTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCA
GATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGA
AAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAAG
CGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSALKRIHKELNDLARDPPAQC
SAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTT
RIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVP
EIARIYKTDREKYNRIAREWTQKYAM*




Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct to express UBE2D2 with N-terminal 6HIS tag (contains Thrombin cleavage site)

Estimated MW of expressed protein : 19786 Daltons
100042 R 1000 1001 1047 19007
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