Ubiquitin | Ubiquitylation Resource

This reagent and more can be found at our sister site MRC PPU Reagents & Services

Request Now

Plasmid

pcDNA5-FRT/TO-DAC-A-C3 monoUbiquitin G75A G76A

Parent Plasmid

pcDNA5 FRT/TO DAC 3C

Expressed

DAC-A-C3 monoUbiquitin G75A G76A

DU Number

DU20569

Genbank

P62988

Tag

DAC

Species

Human

Sequence of Insert

View Sequence

GGATCCGGCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGG
CCGC

Amino Acid Sequence

View Sequence

MIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQA
MKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGIN
LQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADG
QYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSL
NVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLT
WGFRFFETVNPLEVLFQGPGSGAMQIFVKTLTGKTITLEVEPSDTIENVK
AKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRAA**

Vector Type

Mammalian

Antibiotic

Amp

Comments

Flp-In vector for mammalian expression of N-terminally DAC-A tagged G75A G76A ubiquitin (with initiating methionine)

Estimated MW of expressed protein : 38209 Daltons
Parental Plasmid DU30210

100042 R 1000 1001 1047 19007

Download datasheet