UBE2T

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Plasmid
pGEX6P-1-UBE2T K8R K52R K156R
Parent Plasmid
pGEX6P1
Expressed
GST-UBE2T
DU Number
DU20485
Genbank
NP_054895.1
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGCAGAGAGCTTCACGTCTGAGGAGAGAGCTGCACATGTTAGC
CACAGAGCCACCCCCAGGCATCACATGTTGGCAAGATAAAGACCAAATGG
ATGACCTGCGAGCTCAAATATTAGGTGGAGCCAACACACCTTATGAGAAA
GGTGTTTTTAGGCTAGAAGTTATCATTCCTGAGAGGTACCCATTTGAACC
TCCTCAGATCCGATTTCTCACTCCAATTTATCATCCAAACATTGATTCTG
CTGGAAGGATTTGTCTGGATGTTCTCAAATTGCCACCAAAAGGTGCTTGG
AGACCATCCCTCAACATCGCAACTGTGTTGACCTCTATTCAGCTGCTCAT
GTCAGAACCCAACCCTGATGACCCGCTCATGGCTGACATATCCTCAGAAT
TTAAATATAATAAGCCAGCCTTCCTCAAGAATGCCAGACAGTGGACAGAG
AAGCATGCAAGACAGAAACAAAGGGCTGATGAGGAAGAGATGCTTGATAA
TCTACCAGAGGCTGGTGACTCCAGAGTACACAACTCAACACAGAAAAGGA
AGGCCAGTCAGCTAGTAGGCATAGAAAAGAAATTTCATCCTGATGTTTAG
GCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQRASRLRRELHMLATEPP
PGITCWQDKDQMDDLRAQILGGANTPYEKGVFRLEVIIPERYPFEPPQIR
FLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPN
PDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQRADEEEMLDNLPEA
GDSRVHNSTQKRKASQLVGIEKKFHPDV*




Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression construct for GST-UBE2T K8R K52R K156R triple mutant.

Estimated MW of expressed protein : 49430 Daltons
100042 R 1000 1001 1047 19007
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