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Plasmid
pET24a-Ubiquitin-SATGSHHHHHHG
Parent Plasmid
pET24a original
Expressed
Ubiquitin-SATGSHHHHHHG
DU Number
DU21990
Genbank
P62988*
Species
Human
Sequence of Insert
View Sequence
CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAtctgctacaggctctcatcaccatcaccatcacggttgataaggatcc
Amino Acid Sequence
View Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRSATGSHHHHHHG**
Vector Type
Bacterial
Antibiotic
Kan
Comments
Differs from reference sequence in having silent mutation at Arg73 (a220c substitution). Also C-terminal GG removed from ubiquitin. T7 tag in pET24a was removed by subcloning into the Nde1 site, to give untagged protein im the original template construct SC20027.
100042 R 1000 1001 1047 19007
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