Ubiquitin | Ubiquitylation Resource

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Plasmid

pEGFPC4-Ubiquitin G75A G76A octamer

Parent Plasmid

pEGFPC4

Expressed

eGFP-Ubiquitin G75A G76A octamer

DU Number

DU20449

Genbank

P62988*

Tag

GFP

Species

Human

Sequence of Insert

View Sequence

GGATCCGGCGCC ATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCC
TGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAG
AATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCA
GAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTG
ACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGA
GCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGA
AGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACC
ATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGA
CCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCC
TGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGT
CTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCT
TCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGT
GACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCC
TCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGAC
GCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTC
CTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAA
GACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGG
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC
TTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACAT
CCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGC
AGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAG
CCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGG
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG
ATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCAC
CTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSPGSGAM
QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLE
DGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTIEN
VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRA
AMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ
LEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTI
ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRL
RAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAG
KQLEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSD
TIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVL
RLRAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEP
SDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHL
VLRLRAA**

Vector Type

Mammalian

Antibiotic

Kan

Comments

*Each unit differs from ref sequence in having C-terminal AA instead of GG. BamHI-NotI flanked dimer plus STOP codon.
Plasmid for mammalian expression of N-terminally eGFP tagged octo-ubiquitin
Parental Plasmid DU32794

Estimated MW of expressed protein : 96411 Daltons

100042 R 1000 1001 1047 19007

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