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UBE2S

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Plasmid
pET28-UBE2S
Parent Plasmid
pET28a original
Expressed
6His-UBE2S
DU Number
DU20175
Genbank
AAH65364
Tag
6His
Species
Human
Sequence of Insert
GGATCCAACTCCAACGTGGAGAACCTACCCCCGCACATCATCCGCCTGG
TGTACAAGGAGGTGACGACACTGACCGCAGACCCACCCGATGGCATCAAG
GTCTTTCCCAACGAGGAGGACCTCACCGACCTCCAGGTCACCATCGAGGG
CCCTGAGGGGACCCCATATGCTGGAGGTCTGTTCCGCATGAAACTCCTGC
TGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTGACCAAG
ATCTTCCACCCGAACGTGGGCGCCAATGGCGAGATCTGCGTCAACGTGCT
CAAGAGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCA
TCAAGTGCCTGCTGATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAG
GCGGGCCGCCTGCTCTTGGAGAACTACGAGGAGTATGCAGCTCGGGCCCG
TCTGCTCACAGAGATCCACGGGGGCGCCGGCGGGCCCAGCGGCAGGGCCG
AAGCCGGTCGGGCCCTGGCCAGTGGCACTGAAGCTTCCTCCACCGACCCT
GGGGCCCCAGGGGGCCCGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCA
TGCTGGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGA
AGCGGGCGCTGCGGCGGCTGTAGGCGGCCGC
Amino Acid Sequence

HHHHHHSSGLVPRGSHMASMTGGQQMGRGSNSNVENLPPHIIRLVYKEVT
TLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFP
ASPPKGYFLTKIFHPNVGANGEICVNVLKRDWTAELGIRHVLLTIKCLLI
HPNPESALNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGRAL
ASGTEASSTDPGAPGGPGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRR
L*




Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct to express UBE2S with N-terminal 6HIS tag (contains Thrombin cleavage site)

Estimated MW of expressed protein : 27038 Daltons
100042 R 1000 1001 1047 19007
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