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Plasmid
pGEX6P1 UBE2Q1
Parent Plasmid
pGEX6P1
Expressed
GST 3C UBE2Q1
DU Number
DU4213
Genbank
NP_060052
Tag
GST
Species
Human
Sequence of Insert
View Sequence
ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCAGCAG
CTGGGGGGCCAGGGGGCGGCGCCGGGGGCCGGGGGCGGCCCAGGGGGGGG
CCCGGGGCCGGGGCCCTGCCTGAGGCGAGAGCTGAAGCTGCTCGAGTCCA
TCTTCCACCGCGGCCACGAGCGCTTCCGCATTGCCAGCGCCTGCCTGGAC
GAGCTGAGCTGCGAGTTCCTGCTGGCTGGGGCCGGAGGGGCCGGGGCGGG
GGCCGCGCCCGGACCGCATCTCCCCCCACGGGGGTCGGTGCCTGGGGATC
CTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCCCCCC
ATCTGGTCGGTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAG
GCTGGTGGACATAAAGAAAGGGAATACTCTGCTATTGCAGCATCTGAAGA
GGATCATCTCCGACCTGTGTAAACTCTATAACCTCCCTCAGCATCCAGAT
GTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGCACACAGGAAGA
CGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAAGACT
TAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAAATCT
GAAGATGATGGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATTAA
AAAGAACCAGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCGGTGC
AGGCCACTGACCGGCTGATGAAGGAGCTCAGGGATATATACCGATCACAG
AGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAATGACAGTCTGTA
TGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCACA
ACGATCTCCAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCTACTT
AACTTTTCCTTTAAAGATAACTTTCCCTTTGACCCACCATTTGTCAGGGT
TGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGGGCCATCT
GCATGGAACTTCTCACCAAACAGGGCTGGAGCAGTGCCTACTCCATAGAG
TCAGTGATCATGCAGATCAGTGCCACACTGGTGAAGGGGAAAGCACGAGT
GCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAGAGCACAGCAGT
CCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCC
CCAAAAGAAGACGGCTAAGCGGCCGC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCAGCAG
CTGGGGGGCCAGGGGGCGGCGCCGGGGGCCGGGGGCGGCCCAGGGGGGGG
CCCGGGGCCGGGGCCCTGCCTGAGGCGAGAGCTGAAGCTGCTCGAGTCCA
TCTTCCACCGCGGCCACGAGCGCTTCCGCATTGCCAGCGCCTGCCTGGAC
GAGCTGAGCTGCGAGTTCCTGCTGGCTGGGGCCGGAGGGGCCGGGGCGGG
GGCCGCGCCCGGACCGCATCTCCCCCCACGGGGGTCGGTGCCTGGGGATC
CTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCCCCCC
ATCTGGTCGGTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAG
GCTGGTGGACATAAAGAAAGGGAATACTCTGCTATTGCAGCATCTGAAGA
GGATCATCTCCGACCTGTGTAAACTCTATAACCTCCCTCAGCATCCAGAT
GTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGCACACAGGAAGA
CGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAAGACT
TAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAAATCT
GAAGATGATGGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATTAA
AAAGAACCAGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCGGTGC
AGGCCACTGACCGGCTGATGAAGGAGCTCAGGGATATATACCGATCACAG
AGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAATGACAGTCTGTA
TGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCACA
ACGATCTCCAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCTACTT
AACTTTTCCTTTAAAGATAACTTTCCCTTTGACCCACCATTTGTCAGGGT
TGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGGGCCATCT
GCATGGAACTTCTCACCAAACAGGGCTGGAGCAGTGCCTACTCCATAGAG
TCAGTGATCATGCAGATCAGTGCCACACTGGTGAAGGGGAAAGCACGAGT
GCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAGAGCACAGCAGT
CCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCC
CCAAAAGAAGACGGCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFQQPQPQGQQQPGPGQQ
LGGQGAAPGAGGGPGGGPGPGPCLRRELKLLESIFHRGHERFRIASACLD
ELSCEFLLAGAGGAGAGAAPGPHLPPRGSVPGDPVRIHCNITESYPAVPP
IWSVESDDPNLAAVLERLVDIKKGNTLLLQHLKRIISDLCKLYNLPQHPD
VEMLDQPLPAEQCTQEDVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKS
EDDGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQ
SFKGGNYAVELVNDSLYDWNVKLLKVDQDSALHNDLQILKEKEGADFILL
NFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGWSSAYSIE
SVIMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTP
PKEDG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFQQPQPQGQQQPGPGQQ
LGGQGAAPGAGGGPGGGPGPGPCLRRELKLLESIFHRGHERFRIASACLD
ELSCEFLLAGAGGAGAGAAPGPHLPPRGSVPGDPVRIHCNITESYPAVPP
IWSVESDDPNLAAVLERLVDIKKGNTLLLQHLKRIISDLCKLYNLPQHPD
VEMLDQPLPAEQCTQEDVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKS
EDDGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQ
SFKGGNYAVELVNDSLYDWNVKLLKVDQDSALHNDLQILKEKEGADFILL
NFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGWSSAYSIE
SVIMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTP
PKEDG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression plasmid with N terminal GST tag.
100042 R 1000 1001 1047 19007
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