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Plasmid
pGEX6P-2-ATG10
Parent Plasmid
pGEX6P2
Expressed
GST-ATG10
DU Number
DU31144
Genbank
NM_031482.4 GI:196162715
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGAAGAAGATGAGTTCATTGGAGAAAAAACATTCCAACGTTA
TTGTGCAGAATTCATTAAACATTCACAACAGATAGGTGATAGTTGGGAAT
GGAGACCATCAAAGGACTGTTCTGATGGCTACATGTGCAAAATACACTTT
CAAATTAAGAATGGGTCTGTGATGTCACATCTAGGAGCATCTACCCATGG
ACAGACATGTCTTCCCATGGAGGAGGCTTTCGAGCTACCCTTGGATGATT
GTGAAGTGATTGAAACTGCAGCAGCGTCCGAAGTGATTAAATATGAGTAT
CATGTCTTATATTCCTGTAGCTACCAAGTGCCTGTACTTTACTTTAGGGC
AAGCTTTTTAGATGGGAGACCTTTAACTCTGAAGGACATATGGGAAGGAG
TTCATGAGTGCTATAAGATGCGACTGCTACAGGGACCATGGGACACTATT
ACGCAACAGGAACATCCAATACTTGGGCAACCCTTTTTTGTACTTCATCC
CTGCAAGACGAATGAATTCATGACTCCTGTATTAAAGAATTCTCAGAAAA
TCAATAAGAATGTCAACTATATCACATCATGGCTGAGCATTGTAGGGCCA
GTTGTTGGGCTGAATCTACCTCTGAGTTATGCCAAAGCAACGTCTCAGGA
TGAACGAAATGTCCCTTAAGCGGCCGC
TTGTGCAGAATTCATTAAACATTCACAACAGATAGGTGATAGTTGGGAAT
GGAGACCATCAAAGGACTGTTCTGATGGCTACATGTGCAAAATACACTTT
CAAATTAAGAATGGGTCTGTGATGTCACATCTAGGAGCATCTACCCATGG
ACAGACATGTCTTCCCATGGAGGAGGCTTTCGAGCTACCCTTGGATGATT
GTGAAGTGATTGAAACTGCAGCAGCGTCCGAAGTGATTAAATATGAGTAT
CATGTCTTATATTCCTGTAGCTACCAAGTGCCTGTACTTTACTTTAGGGC
AAGCTTTTTAGATGGGAGACCTTTAACTCTGAAGGACATATGGGAAGGAG
TTCATGAGTGCTATAAGATGCGACTGCTACAGGGACCATGGGACACTATT
ACGCAACAGGAACATCCAATACTTGGGCAACCCTTTTTTGTACTTCATCC
CTGCAAGACGAATGAATTCATGACTCCTGTATTAAAGAATTCTCAGAAAA
TCAATAAGAATGTCAACTATATCACATCATGGCTGAGCATTGTAGGGCCA
GTTGTTGGGCTGAATCTACCTCTGAGTTATGCCAAAGCAACGTCTCAGGA
TGAACGAAATGTCCCTTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEEDEFIGEKTFQRYCAEF
IKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCL
PMEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYFRASFLD
GRPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTN
EFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNV
P*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEEDEFIGEKTFQRYCAEF
IKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCL
PMEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYFRASFLD
GRPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTN
EFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNV
P*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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Estimated MW of expressed protein : 52101 Daltons