ZNRF2

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Plasmid
pGEX6P-ZNRF2
Parent Plasmid
pGEX6P1
Expressed
GST-ZNRF2
DU Number
DU16232
Genbank
NM_147128.3 GI:40316919
Tag
GST
Species
Human
Sequence of Insert
ggatccatgggcgccaaacagagcggcccggccgccgctaacggccgcacgcgcgcgtactcgggctcggatctaccttccagtagcagcggaggcgccaatgggaccgcgggcggcggcgggggcgctcgggccgccgccgcggggaggttcccggctcaggtgcccagcgcgcaccagcccagcgcctccggcggcgccgcggcggccgcggcggccccggcagccccggcggccccgcgcagccgctccctcggcggggccgtggggagcgtggcgtcgggggcccgcgcggcgcagtccccc
ttcagcatcccgaacagcagcagcggcccgtacggctcgcaggactcggt
gcacagcagccctgaggacggcggcggcggccgggaccggccggtgggcg
ggagccccggcgggccgcgcctggtgatcggctccttaccagctcacctc
tcgccgcacatgtttggaggatttaagtgccctgtatgctcaaaatttgt
atcctcagatgaaatggatttgcatcttgtaatgtgtttaacaaagccac
gaataacctataatgaggatgtactgagtaaagatgctggggaatgtgca
atatgccttgaagaattgcagcagggagatactatagcacgactgccttg
tctatgcatatatcataaaggctgcatagatgaatggtttgaagtaaata
gatcttgccctgagcacccttcagattaagaattc
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGAKQSGPAAANGRTRAYS
GSDLPSSSSGGANGTAGGGGGARAAAAGRFPAQVPSAHQPSASGGAAAAA
AAPAAPAAPRSRSLGGAVGSVASGARAAQSPFSIPNSSSGPYGSQDSVHS
SPEDGGGGRDRPVGGSPGGPRLVIGSLPAHLSPHMFGGFKCPVCSKFVSS
DEMDLHLVMCLTKPRITYNEDVLSKDAGECAICLEELQQGDTIARLPCLC
IYHKGCIDEWFEVNRSCPEHPSD*

Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial construct to express GST N terminal fusion.

Estimated MW of expressed protein : 50936 Daltons

All ZNRF2 plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
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