UBA6 | Ubiquitylation Resource

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Plasmid

pGEX6P-2-UBA6

Parent Plasmid

pGEX6P2

Expressed

GST-UBA6

DU Number

DU11582

Genbank

NM_018227.5

Tag

GST

Species

Human

Sequence of Insert

View Sequence

gcggccgcgATGGAAGGATCCGAGCCTGTGGCCGCCCATCAGGGGGAAGA
GGCGTCCTGTTCTTCCTGGGGGACTGGCAGCACAAATAAAAATTTGCCCA
TTATGTCAACAGCATCTGTGGAAATCGATGATGCATTGTATAGTCGACAG
AGGTACGTTCTTGGAGACACAGCAATGCAGAAGATGGCCAAGTCCCATGT
TTTCTTAAGTGGGATGGGTGGTCTTGGTTTGGAAATTGCAAAGAATCTTG
TTCTTGCAGGGATTAAGGCAGTTACAATTCATGATACAGAAAAATGCCAA
GCATGGGATCTAGGAACCAACTTCTTTCTCAGTGAAGATGATGTTGTTAA
TAAGAGAAACAGGGCTGAAGCTGTACTTAAACATATTGCAGAACTAAATC
CATACGTTCATGTCACATCATCTTCTGTTCCTTTCAATGAGACCACAGAT
CTCTCCTTTTTAGATAAATACCAGTGTGTAGTATTGACTGAGATGAAACT
TCCATTGCAGAAGAAGATCAATGACTTTTGCCGTTCTCAGTGCCCTCCAA
TTAAGTTTATCAGTGCAGATGTACATGGAATTTGGTCAAGGTTATTTTGT
GATTTCGGTGATGAATTTGAAGTTTTAGATACAACAGGAGAAGAACCAAA
AGAAATTTTCATTTCAAACATAACGCAAGCAAATCCTGGCATTGTTACTT
GCCTTGAAAATCATCCTCACAAACTGGAGACAGGACAATTCCTAACATTT
CGAGAAATTAATGGAATGACAGGTTTAAATGGATCTATACAACAAATAAC
GGTGATATCGCCATTTTCTTTTAGTATTGGTGACACCACAGAACTGGAAC
CATATTTACATGGAGGCATAGCTGTCCAAGTTAAGACTCCTAAAACAGTT
TTTTTTGAATCACTGGAGAGGCAGTTAAAACATCCAAAGTGCCTTATTGT
GGATTTTAGCAACCCTGAGGCACCTTTAGAGATTCACACAGCTATGCTTG
CCTTGGACCAGTTTCAGGAGAAATACAGTCGCAAGCCAAATGTTGGATGC
CAACAAGATTCAGAAGAACTGTTGAAACTAGCAACATCTATAAGTGAAAC
CTTGGAAGAGAAGCCTGATGTAAATGCTGACATTGTGCATTGGCTCTCTT
GGACTGCCCAAGGCTTTTTATCTCCACTTGCTGCAGCAGTAGGAGGTGTT
GCCAGCCAAGAAGTATTGAAAGCTGTAACAGGAAAATTTTCTCCTTTGTG
CCAGTGGTTATATCTTGAAGCAGCAGATATTGTTGAATCACTAGGCAAAC
CTGAATGTGAAGAATTTCTCCCACGAGGAGATAGATATGATGCCTTAAGA
GCTTGCATTGGAGACACTTTGTGTCAGAAACTGCAAAATTTAAACATCTT
CTTAGTAGGGTGTGGAGCCATAGGCTGTGAAATGTTGAAAAATTTTGCTT
TACTTGGTGTTGGCACAAGCAAAGAGAAAGGAATGATTACAGTTACAGAT
CCTGACTTGATAGAGAAATCCAACTTAAATAGACAGTTCCTATTTCGTCC
TCATCACATACAGAAACCTAAAAGCTACACTGCTGCTGATGCTACTCTGA
AAATAAATTCTCAAATAAAGATAGATGCACACCTGAACAAAGTATGTCCA
ACCACTGAGACCATTTACAATGATGAGTTCTATACTAAACAAGATGTAAT
TATTACAGCATTAGATAATGTGGAAGCCAGGAGATACGTAGACAGTCGTT
GCTTAGCAAATCTAAGGCCTCTTTTAGATTCTGGAACAATGGGCACTAAG
GGACACACTGAAGTTATTGTACCGCATTTGACTGAGTCTTACAATAGTCA
TCGGGATCCCCCAGAAGAGGAAATACCATTTTGTACTCTAAAATCCTTTC
CAGCTGCTATTGAACATACCATACAGTGGGCAAGAGATAAGTTTGAAAGT
TCCTTTTCCCACAAACCTTCATTGTTTAACAAATTTTGGCAAACCTATTC
ATCTGCAGAAGAAGTCTTACAGAAGATACAGAGTGGACACAGTTTAGAAG
GCTGTTTTCAAGTTATAAAGTTACTTAGCAGAAGACCTAGAAATTGGTCC
CAGTGTGTAGAATTAGCAAGATTAAAGTTTGAAAAATATTTTAACCATAA
GGCTCTTCAGCTTCTTCACTGTTTCCCTCTGGACATACGATTAAAAGATG
GCAGTTTATTTTGGCAGTCACCAAAGAGGCCACCCTCTCCAATAAAATTT
GATTTAAATGAGCCTTTGCACCTCAGTTTCCTTCAGAATGCTGCAAAACT
ATATGCTACAGTATATTGTATTCCATTTGCAGAAGAGGACTTATCAGCAG
ATGCCCTCTTGAATATTCTTTCAGAAGTAAAGATTCAGGAATTCAAGCCT
TCCAATAAGGTTGTTCAAACAGATGAAACTGCAAGGAAACCAGACCATGT
TCCTATTAGCAGTGAAGATGAGAGGAATGCAATTTTCCAACTAGAAAAGG
CTATTTTATCTAATGAAGCCACCAAAAGTGACCTTCAGATGGCAGTGCTT
TCATTTGAAAAAGATGATGATCATAATGGACACATAGATTTCATCACAGC
TGCATCAAATCTTCGTGCCAAAATGTACAGCATTGAACCAGCTGACCGTT
TCAAAACAAAGCGCATAGCTGGTAAAATTATACCTGCTATAGCAACAACC
ACTGCTACAGTTTCTGGCTTGGTTGCCTTGGAGATGATCAAAGTAACTGG
TGGCTATCCATTTGAAGCTTACAAAAATTGTTTTCTTAACTTAGCCATTC
CAATTGTAGTATTTACAGAGACAACTGAAGTAAGGAAAACTAAAATCAGA
AATGGAATATCATTTACAATTTGGGATCGATGGACCGTACATGGAAAAGA
AGATTTCACCCTCTTGGATTTCATAAATGCAGTCAAAGAGAAGTATGGAA
TTGAGCCAACAATGGTGGTACAGGGAGTCAAAATGCTTTATGTTCCTGTA
ATGCCTGGTCATGCAAAAAGATTGAAGTTAACAATGCATAAACTTGTAAA
ACCTACTACTGAAAAGAAATATGTGGATCTTACTGTGTCATTTGCTCCAG
ACATTGATGGAGATGAAGATTTGCCGGGACCTCCAGTAAGATACTACTTC
AGTCATGACACTGATTAAgcggccgc

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEIPGSTRAAAMEGSEPVA
AHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQK
MAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLS
EDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVV
LTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDT
TGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNG
SIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKH
PKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLA
TSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTG
KFSPLCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKL
QNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNR
QFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFY
TKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLT
ESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNK
FWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFE
KYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFL
QNAAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETA
RKPDHVPISSEDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGH
IDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALE
MIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRW
TVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLT
MHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHDTD*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial construct to express GST N terminal fusion.

Estimated MW of expressed protein : 145840 Daltons

100042 R 1000 1001 1047 19007

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