USP9X

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Plasmid
pEBG6-FLAG-USP9X 1177-2457
Parent Plasmid
pEBG6P
Expressed
GST-FLAG-USP9X 1177-2457
DU Number
DU2863
Genbank
USO90: NM_021906, NM_004652
Tag
GST
FLAG
Species
Human
Sequence of Insert
GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGATGACACAGATCAACCAAGTTACCCATGATCAAGCAGTGGTGCTACAAAGTGCCCTTCAGAGCATTCCTAATCCATCATCCGAGTGCATGCTTAGAAATGTGTCAGTTCGTCTTGCTCAGCAGATATCTGATGAGGCTTCAAGATATATGCCTGATATTTGTGTAATTAGAGCTATACAAAAAATTATCTGGGCATCAGGATGTGGGTCGTTACAGCTAGTATTTAGCCCAAATGAAGAAATCACTAAAATTTATGAGAAGACCAATGCAGGCAATGAGCCAGACTTGGAAGACGAACAGGTTTGCTGTGAAGCATTGGAAGTGATGACCTTATGTTTTGCCTTGATTCCAACAGCCTTAGATGCTCTTAGTAAAGAAAAGGCTTGGCAGACATTCATCATTGACTTACTATTGCACTGTCACAGCAAAACTGTTCGTCAGGTGGCACAGGAGCAGTTCTTTTTAATGTGCACCAGATGTTGCATGGGACACCGGCCTCTACTTTTCTTCATTACTCTACTCTTTACTGTTTTGGGGAGCACAGCAAGAGAGAGAGCTAAACACTCAGGCGACTACTTTACTCTTTTAAGACACCTTCTTAATTACGCTTACAATAGTAATATTAATGTACCCAATGCTGAAGTTCTTCTCAATAATGAAATTGATTGGCTTAAAAGAATTAGGGATGATGTTAAAAGAACAGGAGAAACGGGTATTGAAGAGACGATCTTAGAGGGCCACCTTGGAGTGACAAAGGAGTTACTGGCCTTTCAAACTTCTGAGAAAAAATTTCATATTGGTTGTGAAAAAGGAGGTGCTAATCTCATTAAAGAATTAATTGATGATTTCATATTTCCTGCATCCAATGTTTACCTACAGTATATGAGAAATGGAGAGCTTCCAGCTGAACAGGCTATTCCGGTCTGTGGTTCACCACCTACAATTAATGCTGGTTTTGAATTACTTGTAGCATTAGCTGTTGGCTGTGTGAGGAATCTCAAACAAATAGTAGATTCTTTGACTGAAATGTATTACATTGGCACAGCAATAACTACTTGTGAAGCACTTACTGAGTGGGAATATCTGCCACCTGTTGGACCCCGCCCACCCAAAGGATTCGTGGGGCTGAAAAATGCCGGTGCTACTTGTTACATGAATTCTGTGATTCAGCAACTCTACATGATTCCTTCCATTAGGAACGGTATTCTTGCCATTGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAATGTTGATCCCAGGGATGATGTATTTGGATATCCTCAACAATTTGAAGATAAACCAGCATTAAGTAAAACTGAAGGTAGAAAAGAGTACAACATTGGTGTCCTAAGACACCTTCAGGTCATCTTTGGTCATTTAGCTGCTTCTCGACTGCAATACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTGCGTGAACAACACGATGCTTTAGAATTTTTTAATTCATTGGTGGATAGTTTAGATGAAGCTTTAAAAGCTTTAGGACATCCAGCTATGCTAAGTAAAGTCTTAGGAGGTTCCTTTGCTGATCAGAAGATCTGCCAAGGCCGCCCACATAGGTACGAATGTGAAGAATCTTTTACGACCCTAAACGTAGACATTAGAAATCACCAAAATCTTCTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGGTGCAAATGCATATCATTGTGAAAAATGCAATAAAAAGGTTGATACCGTAAAGCGCTTGCTGATTAAAAAATTACCTCCTGTTCTTGCTATACAACTAAAGCGATTTGACTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTATTTTGAATTTCCTCGAGAGCTGGACATGGAACCTTACACAGTTGCAGGTGTCGCAAAGCTGGAAGGGGATAATGTAAACCCAGAGAGTCAGTTGATACAACAGAGTGAGCAGTCTGAAAGTGAGACAGCAGGAAGCACAAAATACAGACTTGTGGGTGTGCTCGTACACAGTGGTCAAGCGAGTGGGGGGCATTATTATTCTTACATCATCCAAAGGAATGGTGGAGATGGTGAGAGAAATCGCTGGTATAAATTTGATGATGGTGATGTAACAGAATGTAAAATGGATGATGACGAAGAAATGAAAAACCAGTGTTTTGGTGGAGAGTACATGGGAGAAGTGTTTGATCACATGATGAAGCGTATGTCATACAGGCGCCAGAAAAGGTGGTGGAATGCTTATATACTTTTTTATGAACGAATGGACACAATAGACCAAGATGATGAGTTGATAAGATATATATCAGAGCTTGCTATCACCACCAGACCTCATCAGATTATTATGCCATCAGCCATTGAGAGAAGTGTACGGAAACAGAACGTACAATTCATGCATAACCGAATGCAGTACAGTATGGAGTATTTTCAGTTTATGAAAAAACTGCTTACATGTAATGGCGTTTACTTAAACCCTCCTCCCGGGCAAGATCACCTGTTGCCTGAAGCAGAAGAAATCACTATGATCAGTATTCAACTTGCTGCTAGATTCCTCTTTACTACAGGATTTCACACAAAGAAAGTAGTCCGTGGCTCTGCCAGTGATTGGTATGATGCATTGTGTATTCTCCTTCGTCACAGCAAGAATGTACGTTTTTGGTTTGCTCATAACGTCCTTTTTAATGTTTCAAATCGCTTCTCCGAATACCTTCTGGAGTGCCCTAGTGCAGAAGTGAGGGGTGCGTTTGCAAAACTTATAGTCTTTATTGCACATTTTTCCTTGCAAGATGGGCCATGTCCTTCACCTTTTGCCTCTCCTGGACCTTCTAGTCAGGCTTATGACAACTTAAGCTTGAGTGATCACTTACTAAGAGCAGTACTAAATCTCTTGAGAAGGGAAGTTTCAGAGCATGGGCGTCATTTACAGCAGTATTTCAACCTGTTTGTAATGTATGCCAATTTAGGTGTGGCAGAGAAGACACAGCTTCTGAAATTGAGTGTACCTGCTACTTTTATGCTTGTGTCTTTAGATGAAGGTCCAGGTCCTCCAATCAAATACCAGTATGCTGAATTAGGCAAATTATACTCAGTAGTGTCACAGCTGATCCGCTGTTGCAATGTCTCTTCAAGAATGCAGTCTTCAATCAATGGTAATCCTCCTCTTCCCAATCCTTTTGGTGATCCTAATTTATCACAACCTATAATGCCAATTCAGCAGAATGTGGCAGACATTTTATTTGTGAGAACAAGTTATGTGAAGAAAATCATTGAAGACTGCAGTAATTCAGAGGAAACCGTCAAATTGCTTCGTTTTTGCTGCTGGGAGAATCCTCAGTTCTCATCTACTGTCCTCAGTGAACTTCTCTGGCAGGTTGCATATTCCTATACCTATGAACTGCGGCCCTATTTGGATCTGCTTTTGCAAATCTTACTGATTGGGGACTCCTGGCAAACTCACAGGATTCATAATGCACTGAAAGGAATTCCAGATGACCGAGATGGGCTGTTTGACACAATCCAGCGCTCTAAGAATCACTATCAAAAAAGAGCATACCAGTGTATAAAATGTATGGTAGCTCTATTTAGTAACTGTCCTGTTGCTTACCAAATCCTGCAGGGCAATGGAGATCTTAAAAGAAAGTGGACCTGGGCAGTGGAATGGCTTGGAGATGAACTTGAAAGAAGACCATATACTGGCAATCCTCAGTACACTTACAACAATTGGTCTCCCCCAGTGCAAAGCAATGAAACGTCCAATGGTTATTTCTTGGAGAGATCACATAGTGCTAGGATGACACTTGCAAAAGCTTGTGAACTCTGTCCAGAGGAGGAGCCAGATGACCAAGATGCTCCAGATGAACATGAGTCGCCTCCACCTGAAGATGCCCCATTGTACCCCCATTCACCTGGATCTCAGTATCAACAGAATAACCATGTGCATGGACAGCCATATACAGGCCCAGCAGCACATCACATGAACAACCCTCAGAGAACTGGCCAACGAGCACAAGAAAATTATGAAGGCAGTGAAGAAGTATCCCCACCTCAAACCAAGGATCAATGAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSATMDYKDDDDKMTQINQVTHDQAVVLQSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWASGCGSLQLVFSPNEEITKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFALIPTALDALSKEKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAKHSGDYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGETGIEETILEGHLGVTKELLAFQTSEKKFHIGCEKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGSPPTINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQFEDKPALSKTEGRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGRPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDQDDELIRYISELAITTRPHQIIMPSAIERSVRKQNVQFMHNRMQYSMEYFQFMKKLLTCNGVYLNPPPGQDHLLPEAEEITMISIQLAARFLFTTGFHTKKVVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVSSRMQSSINGNPPLPNPFGDPNLSQPIMPIQQNVADILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIGDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHESPPPEDAPLYPHSPGSQYQQNNHVHGQPYTGPAAHHMNNPQRTGQRAQENYEGSEEVSPPQTKDQ*AA
Vector Type
Mammalian
Antibiotic
Amp
Comments
Amplified FROM EST: CD657533 IMAGE:30422528
Changes in this EST compared to NCBI Acc NM_021906

F1393/L
D1628/G
P1944/L
C1720/R
P2323/T
E240/G
MW:185242 D
100042 R 1000 1001 1047 19007
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