UBE2NL | Ubiquitylation Resource

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Plasmid

pET156P UBE2NL

Parent Plasmid

pET15b 6His 3C

Expressed

6His UBE2NL

DU Number

DU15976

Genbank

NP_001013007

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GGATCCGCCGAGCTGCCCCACAGGATCATCAAGGAAACCCAGCGTTTGCT
GGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCC
GTTATTTTCATGTGGTCATTGCTGGGGAATCAAAGGATTCCCCCTTTGAG
GGAGGGACTTTTAAACGTGAACTATTACTTGCAGAAGAATACCCAATGGC
AGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTAGACA
AGTTGGAAAGAATAAGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCA
CTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAATGCTCC
CAATCCAGATGATCCATTAGCAAATGATGTAGTGGAGCAGTGGAAGACCA
ACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCC
ATGAATAGTATTTAAGAATTC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGPGSAELPHRIIKETQRLLAEPVPGIKAEPD
ESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKVRFMTKIYH
PNVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDDPLANDVVE
QWKTNEAQAIETARAWTRLYAMNSI*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter 6HIS for bacterial expression. N.b. numerous differences at DNA level but agrees with UBE2NL protein sequence ref.

Estimated MW of expressed protein : 19689 Daltons

100042 R 1000 1001 1047 19007

Download datasheet