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Plasmid
pGEX6P-1-Ubiquitin K48wt K63wt
Parent Plasmid
pGEX6P1
Expressed
GST-Ubiquitin K48wt K63wt
DU Number
DU8727
Genbank
P62988
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCTCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCG
AAGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGAC
AGGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACA
GCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCA
CCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGACTCGAGC
GGCCGC
AAGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGAC
AGGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACA
GCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCA
CCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGACTCGAGC
GGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVRTLTGRTITLEVEPS
DTIENVRARIQDREGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVRTLTGRTITLEVEPS
DTIENVRARIQDREGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST for bacterial expression.
100042 R 1000 1001 1047 19007
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