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USP7

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Plasmid
pEBG6P-USP7 1-525
Parent Plasmid
pEBG6P
Expressed
GST USP7 1-525
DU Number
DU32691
Genbank
NP_003461.2
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGAACCACCAGCAGCAGCAGCAGCAGCAGAAAGCGGGCGAGCA
GCAGTTGAGCGAGCCCGAGGACATGGAGATGGAAGCGGGAGATACAGATG
ACCCACCAAGAATTACTCAGAACCCTGTGATCAATGGGAATGTGGCCCTG
AGTGATGGACACAACACCGCGGAGGAGGACATGGAGGATGACACCAGTTG
GCGCTCCGAGGCAACCTTTCAGTTCACTGTGGAGCGCTTCAGCAGACTGA
GTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGCGAAATCTGCCATGGAAG
ATTATGGTGATGCCACGCTTTTATCCAGACAGACCACACCAAAAAAGCGT
AGGATTCTTTCTCCAGTGCAATGCTGAATCTGATTCCACGTCATGGTCTT
GCCATGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGATGAAAAG
TCGTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGATTG
GGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGAAAG
GATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCGGAT
GCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGTCGG
CTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGACGT
TATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACCGAG
GGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTTCTA
TGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAAAGT
CATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAGGAG
CTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCACCTG
TGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCTATA
TCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTATTAT
GATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATTTGT
GGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACGCTG
GGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACATTG
CCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCAGAC
GGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGTTAC
CACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAATTAT
ATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACATTA
TGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTGATG
ACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAATTAT
GGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTACAT
GTTAGTCTACATCAGGGAATCAAAACTGAGTTAAGTTTTACAGGCGGTCA
CCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAGAAA
AGGATCTAGGCTCAGAAGCGGAAGGAGCGGCAGGAAGCCCATCTCTATAT
GCAAGTGCAGATAGTCGCAGAGGACCAGTTTTGTGGCCACCAAGGGAATG
ACATGTACGATGAAGAAAAAGTGAAATACACTGTGTTCAAAGTATTGAAG
AACTCCTCGCTTGCTGAGTTTGTTCAGAGCCTCTCTCAGACCATGGGATT
TCCACAAGATCAAATTCGATTGTGGCCCATGCAAGCAAGGAGTAATGGAA
CAAAACGACCAGCAATGTTAGATAATGAAGCCGACGGCAATAAAACAATG
ATTGAGCTCAGTGATAATGAAAACCCTTGGACAATATTCCTGGAAACAGT
TGATCCCGAGCTGGCTGCTAGTGGAGCGACCTTACCCAAGTTTGATAAAG
ATCATGATGTAATGTTATTTTTGAAGATGTATGATCCCAAAACGCGGAGC
TTGAATTACTGTGGGCATATCTACACACCAATATCCTGTAAAATACGTGA
CTTGCTCCCAGTTATGTGTGACAGAGCAGGATTTATTCAAGATACTAGCC
TTATCCTCTATGAGGAAGTTAAACCGAATTTAACAGAGAGAATTCAGGAC
TATGACGTGTCTCTTGATAAAGCCCTTGATGAACTAATGGATGGTGACAT
CATAGTATTTCAGAAGGATGACCCTGAAAATGATAACAGTGAATTACCCA
CCGCAAAGGAGTATTTCCGAGATCTCTACCACCGCGTTGATGTCATTTTC
TGTGATAAAACAATCCCTAATGATCCTGGATTTGTGGTTACGTTATCAAA
TAGAATGAATTATTTTCAGGTTGCAAAGACAGTTGCACAGAGGCTCAACA
CAGATCCAATGTTGCTGCAGTTTTTCAAGTCTCAAGGTTATAGGGATGGC
CCAGGTAATCCTCTTAGACATAATTATGAAGGTACTTTAAGAGATCTTCT
ACAGTTCTTCAAGCCTAGACAACCTAAGAAACTTTACTATCAGCAGCTTA
AGATGAAAATCACAGACTTTGAGAACAGGCGAAGTTTTAAATGTATATGG
TTAAACAGCCAATTTAGGGAAGAGGAAATAACACTATATCCAGACAAGCA
TGGGTGTGTCCGGGACCTGTTAGAAGAATGTAAAAAGGCCGTGGAGCTTG
GGGAGAAAGCATCAGGGAAACTTAGGCTGCTAGAAATTGTAAGCTACAAA
ATCATTGGTGTTCATCAAGAAGATGAACTATTAGAATGTTTATCTCCTGC
AACGAGCCGGACGTTTCGAATAGAGGAAATCCCTTTGGACCAGGTGGACA
TAGACAAAGAGAATGAGATGCTTGTCACAGTGGCGCATTTCCACAAAGAG
GTCTTCGGAACGTTCGGAATCCCGTTTTTGCTGAGGATACACCAGGGCGA
GCATTTTCGAGAAGTGATGAAGCGAATCCAGAGCCTGCTGGACATCCAGG
AGAAGGAGTTTGAGAAGTTTAAATTTGCAATTGTAATGATGGGCCGACAC
CAGTACATAAATGAAGACGAGTATGAAGTAAATTTGAAAGACTTTGAGCC
ACAGCCCGGTAATATGTCTCATCCTCGGCCTTGGCTAGGGCTCGACCACT
TCAACAAAGCCCCAAAGAGGAGTCGCTACACTTACCTTGAAAAGGCCATT
AAAATCCATAACTGAGCGGCCGC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMNHQQQQQQQKAG
EQQLSEPEDMEMEAGDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDT
SWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQK
SVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKEN
DWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGY
VGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRV
FYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKG
TCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFES
FVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDP
QTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGG
HYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNA
YMLVYIRESKLS*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter GST for mammalian expression

Estimated MW of expressed protein : 88003 Daltons
100042 R 1000 1001 1047 19007
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