TRAF3 | Ubiquitylation Resource

Plasmid

pCMV-FLAG-1 TRAF3 88-568

Parent Plasmid

pCMV5 FLAG1

Expressed

FLAG TRAF3 88-568

DU Number

DU32805

Genbank

AAH75086.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCTGTACAGCGTGTCAAGAGAGCATCGTTAAAGATAAGGTGTTTAA
GGATAATTGCTGCAAGAGAGAAATTCTGGCTCTTCAGATCTATTGTCGGA
ATGAAAGCAGAGGTTGTGCAGAGCAGTTAATGCTGGGACATCTGCTGGTG
CATTTAAAAAATGATTGCCATTTTGAAGAACTTCCATGTGTGCGTCCTGA
CTGCAAAGAAAAGGTCTTGAGGAAAGACCTGCGAGACCACGTGGAGAAGG
CGTGTAAATACCGGGAAGCCACATGCAGCCACTGCAAGAGTCAGGTTCCG
ATGATCGCGCTGCAGAAACACGAAGACACCGACTGTCCCTGCGTGGTGGT
GTCCTGCCCTCACAAGTGCAGCGTCCAGACTCTCCTGAGGAGCGAGTTGA
GTGCACACTTGTCAGAGTGTGTCAATGCCCCCAGCACCTGTAGTTTTAAG
CGCTATGGCTGCGTTTTTCAGGGGACAAACCAGCAGATCAAGGCCCACGA
GGCCAGCTCCGCCGTGCAGCACGTCAACCTGCTGAAGGAGTGGAGCAACT
CGCTCGAAAAGAAGGTTTCCTTGTTGCAGAATGAAAGTGTAGAAAAAAAC
AAGAGCATACAAAGTTTGCACAATCAGATATGTAGCTTTGAAATTGAAAT
TGAGAGACAAAAGGAAATGCTTCGAAATAATGAATCCAAAATCCTTCATT
TACAGCGAGTGATAGACAGCCAAGCAGAGAAACTGAAGGAGCTTGACAAG
GAGATCCGGCCCTTCCGGCAGAACTGGGAGGAAGCAGACAGCATGAAGAG
CAGCGTGGAGTCCCTCCAGAACCGCGTGACCGAGCTGGAGAGCGTGGACA
AGAGCGCGGGGCAAGTGGCTCGGAACACAGGCCTGCTGGAGTCCCAGCTG
AGCCGGCATGACCAGATGCTGAGTGTGCACGACATCCGCCTAGCCGACAT
GGACCTGCGCTTCCAGGTCCTGGAGACCGCCAGCTACAATGGAGTGCTCA
TCTGGAAGATTCGCGACTACAAGCGGCGGAAGCAGGAGGCCGTCATGGGG
AAGACCCTGTCCCTTTACAGCCAGCCTTTCTACACTGGTTACTTTGGCTA
TAAGATGTGTGCCAGGGTCTACCTGAACGGGGACGGGATGGGGAAGGGGA
CGCACTTGTCGCTGTTTTTTGTCATCATGCGTGGAGAATATGATGCCCTG
CTTCCTTGGCCGTTTAAGCAGAAAGTGACACTCATGCTGATGGATCAGGG
GTCCTCTCGACGTCATTTGGGAGATGCATTCAAGCCCGACCCCAACAGCA
GCAGCTTCAAGAAGCCCACTGGAGAGATGAATATCGCCTCTGGCTGCCCA
GTCTTTGTGGCCCAAACTGTTCTAGAAAATGGGACATATATTAAAGATGA
TACAATTTTTATTAAAGTCATAGTGGATACTTCGGATCTGCCCGATCCCT
GACTCGAG

Amino Acid Sequence

View Sequence

MDYKDDDDKGSCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAE
QLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREAT
CSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECV
NAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSL
LQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQ
AEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVAR
NTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYK
RRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFV
IMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTG
EMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalin expression

Estimated MW of expressed protein : 56184 Daltons

100042 R 1000 1001 1047 19007