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A20

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Plasmid
pFBHTb A20bn
Parent Plasmid
pFastBac HTb
Expressed
6His A20
DU Number
DU35296
Genbank
BC114480
Tag
6His
Species
Human
Sequence of Insert
GCGGCCGCGATGGCTGAACAAGTCCTTCCTCAGGCTTTGTATTTGAGCAA
TATGCGGAAAGCTGTGAAGATACGGGAGAGAACTCCAGAAGACATTTTTA
AACCTACTAATGGGATCATTCATCATTTTAAAACCATGCACCGATACACA
CTGGAAATGTTCAGAACTTGCCAGTTTTGTCCTCAGTTTCGGGAGATCAT
CCACAAAGCCCTCATCGACAGAAACATCCAGGCCACCCTGGAAAGCCAGA
AGAAACTCAACTGGTGTCGAGAAGTCCGGAAGCTTGTGGCGCTGAAAACG
AACGGTGACGGCAATTGCCTCATGCATGCCACTTCTCAGTACATGTGGGG
CGTTCAGGACACAGACTTGGTACTGAGGAAGGCGCTGTTCAGCACGCTCA
AGGAAACAGACACACGCAACTTTAAATTCCGCTGGCAACTGGAGTCTCTC
AAATCTCAGGAATTTGTTGAAACGGGGCTTTGCTATGATACTCGGAACTG
GAATGATGAATGGGACAATCTTATCAAAATGGCTTCCACAGACACACCCA
TGGCCCGAAGTGGACTTCAGTACAACTCACTGGAAGAAATACACATATTT
GTCCTTTGCAACATCCTCAGAAGGCCAATCATTGTCATTTCAGACAAAAT
GCTAAGAAGTTTGGAATCAGGTTCCAATTTCGCCCCTTTGAAAGTGGGTG
GAATTTACTTGCCTCTCCACTGGCCTGCCCAGGAATGCTACAGATACCCC
ATTGTTCTCGGCTATGACAGCCATCATTTTGTACCCTTGGTGACCCTGAA
GGACAGTGGGCCTGAAATCCGAGCTGTTCCACTTGTTAACAGAGACCGGG
GAAGATTTGAAGACTTAAAAGTTCACTTTTTGACAGATCCTGAAAATGAG
ATGAAGGAGAAGCTCTTAAAAGAGTACTTAATGGTGATAGAAATCCCCGT
CCAAGGCTGGGACCATGGCACAACTCATCTCATCAATGCCGCAAAGTTGG
ATGAAGCTAACTTACCAAAAGAAATCAATCTGGTAGATGATTACTTTGAA
CTTGTTCAGCATGAGTACAAGAAATGGCAGGAAAACAGCGAGCAGGGGAG
GAGAGAGGGGCACGCCCAGAATCCCATGGAACCTTCCGTGCCCCAGCTTT
CTCTCATGGATGTAAAATGTGAAACGCCCAACTGCCCCTTCTTCATGTCT
GTGAACACCCAGCCTTTATGCCATGAGTGCTCAGAGAGGCGGCAAAAGAA
TCAAAACAAACTCCCAAAGCTGAACTCCAAGCCGGGCCCTGAGGGGCTCC
CTGGCATGGCGCTCGGGGCCTCTCGGGGAGAAGCCTATGAGCCCTTGGCG
TGGAACCCTGAGGAGTCCACTGGGGGGCCTCATTCGGCCCCACCGACAGC
ACCCAGCCCTTTTCTGTTCAGTGAGACCACTGCCATGAAGTGCAGGAGCC
CCGGCTGCCCCTTCACACTGAATGTGCAGCACAACGGATTTTGTGAACGT
TGCCACAACGCCCGGCAACTTCACGCCAGCCACGCCCCAGACCACACAAG
GCACTTGGATCCCGGGAAGTGCCAAGCCTGCCTCCAGGATGTTACCAGGA
CATTTAATGGGATCTGCAGTACTTGCTTCAAAAGGACTACAGCAGAGGCC
TCCTCCAGCCTCAGCACCAGCCTCCCTCCTTCCTGTCACCAGCGTTCCAA
GTCAGATCCCTCGCGGCTCGTCCGGAGCCCCTCCCCGCATTCTTGCCACA
GAGCTGGAAACGACGCCCCTGCTGGCTGCCTGTCTCAAGCTGCACGGACT
CCTGGGGACAGGACGGGGACGAGCAAGTGCAGAAAAGCCGGCTGCGTGTA
TTTTGGGACTCCAGAAAACAAGGGCTTTTGCACACTGTGTTTCATCGAGT
ACAGAGAAAACAAACATTTTGCTGCTGCCTCAGGGAAAGTCAGTCCCACA
GCGTCCAGGTTCCAGAACACCATTCCGTGCCTGGGGAGGGAATGCGGCAC
CCTTGGAAGCACCATGTTTGAAGGATACTGCCAGAAGTGTTTCATTGAAG
CTCAGAATCAGAGATTTCATGAGGCCAAAAGGACAGAAGAGCAACTGAGA
TCGAGCCAGCGCAGAGATGTGCCTCGAACCACACAAAGCACCTCAAGGCC
CAAGTGCGCCCGGGCCTCCTGCAAGAACATCCTGGCCTGCCGCAGCGAGG
AGCTCTGCATGGAGTGTCAGCATCCCAACCAGAGGATGGGCCCTGGGGCC
CACCGGGGTGAGCCTGCCCCCGAAGACCCCCCCAAGCAGCGTTGCCGGGC
CCCCGCCTGTGATCATTTTGGCAATGCCAAGTGCAACGGCTACTGCAACG
AATGCTTTCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC
Amino Acid Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMAEQVLP
QALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMHRYTLEMFRTCQFC
PQFREIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGNCLMHA
TSQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGL
CYDTRNWNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPI
IVISDKMLRSLESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHF
VPLVTLKDSGPEIRAVPLVNRDRGRFEDLKVHFLTDPENEMKEKLLKEYL
MVIEIPVQGWDHGTTHLINAAKLDEANLPKEINLVDDYFELVQHEYKKWQ
ENSEQGRREGHAQNPMEPSVPQLSLMDVKCETPNCPFFMSVNTQPLCHEC
SERRQKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNPEESTGGP
HSAPPTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHAS
HAPDHTRHLDPGKCQACLQDVTRTFNGICSTCFKRTTAEASSSLSTSLPP
SCHQRSKSDPSRLVRSPSPHSCHRAGNDAPAGCLSQAARTPGDRTGTSKC
RKAGCVYFGTPENKGFCTLCFIEYRENKHFAAASGKVSPTASRFQNTIPC
LGRECGTLGSTMFEGYCQKCFIEAQNQRFHEAKRTEEQLRSSQRRDVPRT
TQSTSRPKCARASCKNILACRSEELCMECQHPNQRMGPGAHRGEPAPEDP
PKQRCRAPACDHFGNAKCNGYCNECFQFKQMYG*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
N ter 6HIS for baculovirus expression

Estimated MW of expressed protein : 94423 Daltons
100042 R 1000 1001 1047 19007
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