USP28 | Ubiquitylation Resource

Plasmid

pcDNA5-FRT/TO-FLAG USP28 1-582

Parent Plasmid

pcDNA5 FRT/TO FLAG1

Expressed

FLAG-USP28

DU Number

DU12777

Genbank

AAH65928.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGACTGCGGAGCTGCAGCAGGACGACGCGGCCGGCGCGGCAGA
CGGCCACGGCTCGAGCTGCCAAATGCTGTTAAATCAACTGAGAGAAATCA
CAGGCATTCAGGACCCTTCCTTTCTCCATGAAGCTCTGAAGGCCAGTAAT
GGTGACATTACTCAGGCAGTCAGCCTTCTCACTGATGAGAGAGTTAAGGA
GCCCAGTCAAGACACTGTTGCTACAGAACCATCTGAAGTAGAGGGGAGTG
CTGCCAACAAGGAAGTATTAGCAAAAGTTATAGACCTTACTCATGATAAC
AAAGATGATCTTCAGGCTGCCATTGCTTTGAGTCTACTGGAGTCTCCCAA
AATTCAAGCTGATGGAAGAGATCTTAACAGGATGCATGAAGCAACCTCTG
CAGAAACTAAACGCTCAAAGAGAAAACGCTGTGAAGTCTGGGGAGAAAAC
CCCAATCCCAATGACTGGAGGAGAGTTGATGGTTGGCCAGTTGGGCTGAA
AAATGTTGGCAATACATGTTGGTTTAGTGCTGTTATTCAGTCTCTCTTTC
AATTGCCTGAATTTCGAAGACTTGTTCTCAGTTATAGTCTGCCACAAAAT
GTACTTGAAAATTGTCGAAGTCATACAGAAAAGAGAAATATCATGTTTAT
GCAAGAGCTTCAGTATTTGTTTGCTCTAATGATGGGATCAAATAGAAAAT
TTGTAGACCCGTCTGCAGCCCTGGATCTATTAAAGGGAGCATTCCGATCA
TCTGAGGAACAGCAGCAAGATGTGAGTGAATTCACACACAAGCTCCTGGA
TTGGCTAGAGGACGCATTCCAGCTAGCTGTTAATGTTAACAGTCCCAGGA
ACAAATCTGAAAATCCAATGGTGCAGCTGTTCTATGGTACTTTCCTGACT
GAAGGGGTTCGTGAAGGAAAACCCTTTTGTAACAATGAGACCTTCGGCCA
GTATCCTCTTCAGGTAAACGGTTATCGCAACTTAGACGAGTGTTTGGAAG
GGGCCATGGTGGAGGGTGATGTTGAGCTTCTTCCCTCCGATCACTCGGTG
AAGTATGGACAAGAGCGTTGGTTTACAAAGCTACCTCCAGTGTTGACCTT
TGAACTCTCAAGATTTGAGTTTAATCAGTCCCTTGGGCAGCCAGAGAAAA
TTCACAATAAGCTGGAATTTCCTCAGATTATTTATATGGACAGGTACATG
TACAGGAGCAAGGAGCTTATTCGAAATAAGAGAGAGTGTATTCGAAAGTT
GAAGGAGGAAATAAAAATTCTGCAGCAAAAATTGGAAAGGTATGTGAAAT
ATGGCTCAGGCCCAGCTCGGTTCCCGCTCCCGGACATGCTGAAATATGTT
ATTGAATTTGCTAGTACAAAACCTGCCTCAGAAAGCTGTCCACCTGAAAG
TGACACACATATGACATTACCACTTTCTTCAGTGCACTGCTCGGTTTCTG
ACCAGACATCCAAGGAAAGTACAAGTACAGAAAGCTCTTCTCAGGATGTT
GAAAGTACCTTTTCTTCTCCTGAAGATTCTTTACCCAAGTCTAAACCACT
GACATCTTCTCGGTCTTCCATGGAAATGCCTTCACAGCCAGCTCCACGAA
CAGTCACAGATGAGGAGATAAATTTTGTTAAGACCTGTCTTCAGAGATGG
AGGAGTGAGATTGAACAAGATATACAAGATTTAAAGACTTGTATTGCAAG
TACTACTCAGACTATTGAACAGATGTACTGCGATCCTCTCCTTCGTCAGG
TAGAATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSF
LHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLA
KVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKR
KRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRL
VLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAAL
DLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMV
QLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDV
ELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFP
QIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARF
PLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKEST
STESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEIN
FVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVE*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalian expression.

Estimated MW of expressed protein : 67511 Daltons

100042 R 1000 1001 1047 19007