TRAF3 | Ubiquitylation Resource

Plasmid

pBabe.puro-FLAG TRAF3

Parent Plasmid

pBABE puro FLAG

Expressed

FLAG TRAF3

DU Number

DU32659

Genbank

AAH75086.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGAGTCGAGTAAAAAGATGGACTCTCCTGGCGCGCTGCAGAC
TAACCCGCCGCTAAAGCTGCACACTGACCGCAGTGCTGGGACGCCAGTTT
TTGTCCCTGAACAAGGAGGTTACAAGGAAAAGTTTGTGAAGACCGTGGAG
GACAAGTACAAGTGTGAGAAGTGCCACCTGGTGCTGTGCAGCCCGAAGCA
GACCGAGTGTGGGCACCGCTTCTGCGAGAGCTGCATGGCGGCCCTGCTGA
GCTCTTCAAGTCCAAAATGTACAGCGTGTCAAGAGAGCATCGTTAAAGAT
AAGGTGTTTAAGGATAATTGCTGCAAGAGAGAAATTCTGGCTCTTCAGAT
CTATTGTCGGAATGAAAGCAGAGGTTGTGCAGAGCAGTTAATGCTGGGAC
ATCTGCTGGTGCATTTAAAAAATGATTGCCATTTTGAAGAACTTCCATGT
GTGCGTCCTGACTGCAAAGAAAAGGTCTTGAGGAAAGACCTGCGAGACCA
CGTGGAGAAGGCGTGTAAATACCGGGAAGCCACATGCAGCCACTGCAAGA
GTCAGGTTCCGATGATCGCGCTGCAGAAACACGAAGACACCGACTGTCCC
TGCGTGGTGGTGTCCTGCCCTCACAAGTGCAGCGTCCAGACTCTCCTGAG
GAGCGAGTTGAGTGCACACTTGTCAGAGTGTGTCAATGCCCCCAGCACCT
GTAGTTTTAAGCGCTATGGCTGCGTTTTTCAGGGGACAAACCAGCAGATC
AAGGCCCACGAGGCCAGCTCCGCCGTGCAGCACGTCAACCTGCTGAAGGA
GTGGAGCAACTCGCTCGAAAAGAAGGTTTCCTTGTTGCAGAATGAAAGTG
TAGAAAAAAACAAGAGCATACAAAGTTTGCACAATCAGATATGTAGCTTT
GAAATTGAAATTGAGAGACAAAAGGAAATGCTTCGAAATAATGAATCCAA
AATCCTTCATTTACAGCGAGTGATAGACAGCCAAGCAGAGAAACTGAAGG
AGCTTGACAAGGAGATCCGGCCCTTCCGGCAGAACTGGGAGGAAGCAGAC
AGCATGAAGAGCAGCGTGGAGTCCCTCCAGAACCGCGTGACCGAGCTGGA
GAGCGTGGACAAGAGCGCGGGGCAAGTGGCTCGGAACACAGGCCTGCTGG
AGTCCCAGCTGAGCCGGCATGACCAGATGCTGAGTGTGCACGACATCCGC
CTAGCCGACATGGACCTGCGCTTCCAGGTCCTGGAGACCGCCAGCTACAA
TGGAGTGCTCATCTGGAAGATTCGCGACTACAAGCGGCGGAAGCAGGAGG
CCGTCATGGGGAAGACCCTGTCCCTTTACAGCCAGCCTTTCTACACTGGT
TACTTTGGCTATAAGATGTGTGCCAGGGTCTACCTGAACGGGGACGGGAT
GGGGAAGGGGACGCACTTGTCGCTGTTTTTTGTCATCATGCGTGGAGAAT
ATGATGCCCTGCTTCCTTGGCCGTTTAAGCAGAAAGTGACACTCATGCTG
ATGGATCAGGGGTCCTCTCGACGTCATTTGGGAGATGCATTCAAGCCCGA
CCCCAACAGCAGCAGCTTCAAGAAGCCCACTGGAGAGATGAATATCGCCT
CTGGCTGCCCAGTCTTTGTGGCCCAAACTGTTCTAGAAAATGGGACATAT
ATTAAAGATGATACAATTTTTATTAAAGTCATAGTGGATACTTCGGATCT
GCCCGATCCCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGY
KEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCT
ACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKN
DCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIAL
QKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGC
VFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQ
SLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRP
FRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHD
QMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLS
LYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWP
FKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVA
QTVLENGTYIKDDTIFIKVIVDTSDLPDP*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N Ter FLAG for mammalian expression

Estimated MW of expressed protein : 65759 Daltons

100042 R 1000 1001 1047 19007