TRIM25

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Plasmid
pGEX6P-2-TRIM 25
Parent Plasmid
pGEX6P2
Expressed
GST-TRIM 25
DU Number
DU12449
Genbank
NP_005073.2
Tag
GST
Species
Human
Sequence of Insert
GCGGCCGCGATGGCAGAGCTGTGCCCCCTGGCCGAGGAGCTGTCGTGCTC
CATCTGCCTGGAGCCCTTCAAGGAGCCGGTCACCACTCCGTGCGGCCACA
ACTTCTGCGGGTCGTGCCTGAATGAGACGTGGGCAGTCCAGGGCTCGCCA
TACCTGTGCCCGCAGTGCCGCGCCGTCTACCAGGCGCGACCGCAGCTGCA
CAAGAACACGGTGCTGTGCAACGTGGTGGAGCAGTTCCTGCAGGCCGACC
TGGCCCGGGAGCCACCCGCCGACGTCTGGACGCCGCCCGCCCGCGCCTCT
GCACCCAGCCCGAATGCCCAGGTGGCCTGCGACCACTGCCTGAAGGAGGC
CGCCGTGAAGACGTGCTTGGTGTGCATGGCCTCCTTCTGTCAGGAGCACC
TGCAGCCGCACTTCGACAGCCCCGCCTTCCAGGACCACCCGCTGCAGCCG
CCCGTTCGCGACCTGTTGCGCCGCAAATGTTCCCAGCACAATCGGCTGCG
GGAATTTTTCTGCCCCGAGCACAGCGAGTGCATCTGCCACATCTGCCTGG
TGGAGCATAAGACCTGCTCTCCCGCGTCCCTGAGCCAGGCCAGCGCCGAC
CTGGAGGCCACCCTGAGGCACAAACTAACTGTCATGTACAGTCAGATCAA
CGGGGCGTCGAGAGCACTGGATGATGTGAGAAACAGGCAGCAGGATGTGC
GGATGACTGCAAACAGAAAGGTGGAGCAGCTACAACAAGAATACACGGAA
ATGAAGGCTCTCTTGGACGCCTCAGAGACCACCTCGACAAGGAAGATAAA
GGAAGAGGAGAAGAGGGTCAACAGCAAGTTTGACACCATTTATCAGATTC
TCCTCAAGAAGAAGAGTGAGATCCAGACCTTGAAGGAGGAGATTGAACAG
AGCCTGACCAAGAGGGATGAGTTCGAGTTTCTGGAGAAAGCATCAAAACT
GCGAGGAATCTCAACAAAGCCAGTCTACATCCCCGAGGTGGAACTGAACC
ACAAGCTGATAAAAGGCATCCACCAGAGCACCATAGACCTCAAAAACGAG
CTGAAGCAGTGCATCGGGCGGCTCCAGGAGCTCACCCCCAGTTCAGGTGA
CCCTGGAGAGCATGACCCAGCGTCCACACACAAATCCACACGCCCTGTGA
AGAAGGTCTCCAAAGAGGAAAAGAAATCCAAGAAACCTCCCCCTGTCCCT
GCCTTACCCAGCAAGCTTCCCACGTTTGGAGCCCCGGAACAGTTAGTGGA
TTTAAAACAAGCTGGCTTGGAGGCTGCAGCCAAAGCCACCAGCTCACATC
CGAACTCAACATCTCTCAAGGCCAAGGTGCTGGAGACCTTCCTGGCCAAG
TCCAGACCTGAGCTCCTGGAGTATTACATTAAAGTCATCCTGGACTACAA
CACCGCCCACAACAAAGTGGCTCTGTCAGAGTGCTATACAGTAGCTTCTG
TGGCTGAGATGCCTCAGAACTACCGGCCGCATCCCCAGAGGTTCACATAC
TGCTCTCAGGTGCTGGGCCTGCACTGCTACAAGAAGGGGATCCACTACTG
GGAGGTGGAGCTGCAGAAGAACAACTTCTGTGGGGTAGGCATCTGCTACG
GAAGCATGAACCGGCAGGGCCCAGAAAGCAGGCTCGGCCGCAACAGCGCC
TCCTGGTGCGTGGAGTGGTTCAACACCAAGATCTCTGCCTGGCACAATAA
CGTGGAGAAAACCCTGCCCTCCACCAAGGCCACGCGGGTGGGCGTGCTTC
TCAACTGTGACCACGGCTTTGTCATCTTCTTCGCTGTTGCCGACAAGGTC
CACCTGATGTATAAGTTCAGGGTGGACTTTACTGAGGCTTTGTACCCGGC
TTTCTGGGTATTTTCTGCTGGTGCCACACTCTCCATCTGCTCCCCCAAGT
AGGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAELCPLA
EELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQ
ARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACD
HCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCS
QHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTV
MYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETT
STRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFL
EKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEL
TPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGA
PEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIK
VILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYK
KGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKI
SAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFT
EALYPAFWVFSAGATLSICSPK*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter GST for bacterial expression.

Estimated MW of expressed protein : 98789 Daltons
100042 R 1000 1001 1047 19007
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