TRIM28 | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-TAP TRIM28

Parent Plasmid

pCDNA5 FRT/TO TAP

Expressed

TAP TRIM28

DU Number

DU15120

Genbank

NP_005753.1

Tag

TAP

Species

Human

Sequence of Insert

View Sequence

GCGGCCGCGATGGCGGCCTCCGCGGCGGCAGCCTCGGCAGCAGCGGCCTC
GGCCGCCTCTGGCAGCCCGGGCCCGGGCGAGGGCTCCGCTGGCGGCGAAA
AGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGCCTCAGCCGCG
GCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCA
CTGCGGCGTGTGCAGAGAGCGCCTGCGACCCGAGAGGGAGCCCCGCCTGC
TGCCCTGTTTGCACTCGGCCTGTAGTGCCTGCTTAGGGCCCGCGGCCCCC
GCCGCCGCCAACAGCTCGGGGGACGGCGGGGCGGCGGGCGACGGCACCGT
GGTGGACTGTCCCGTGTGCAAGCAACAGTGCTTCTCCAAAGACATCGTGG
AGAATTATTTCATGCGTGATAGTGGCAGCAAGGCTGCCACCGACGCCCAG
GATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCAG
CTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGC
ACCAGCGGGTGAAGTACACCAAGGACCATACTGTGCGCTCTACTGGGCCA
GCCAAGTCTCGGGATGGTGAACGTACTGTCTATTGCAACGTACACAAGCA
TGAACCCCTTGTGCTGTTTTGTGAGAGCTGTGATACTCTCACCTGCCGAG
ACTGCCAGCTCAATGCCCACAAGGACCACCAGTACCAGTTCTTAGAGGAT
GCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGG
GGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAA
TCCGCCAGGTGTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATG
GCCATCCTGCAGATCATGAAGGAGCTGAATAAGCGGGGCCGTGTGCTGGT
CAATGATGCCCAGAAGGTGACTGAGGGGCAGCAGGAGCGCCTGGAGCGGC
AGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCACATTCTGCGC
TTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTC
TAAGAAGTTGATCTACTTCCAGCTGCACCGGGCCCTCAAGATGATTGTGG
ATCCCGTGGAGCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGCC
TGGACCAAGAGTGCCGAGGCCTTTGGCAAGATTGTGGCAGAGCGTCCTGG
CACTAACTCAACAGGCCCTGCACCCATGGCCCCTCCAAGAGCCCCAGGGC
CCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGGAGGTGCAGGAA
GGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCA
TGTGTCAGGTGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCC
TTATGCGCAAGGTGCCACGAGTGAGCCTTGAACGCCTGGACCTGGACCTC
ACAGCTGACAGCCAGCCACCCGTCTTCAAGGTCTTCCCAGGCAGTACCAC
TGAGGACTACAACCTTATTGTTATTGAACGTGGCGCTGCCGCTGCAGCTA
CCGGCCAGCCAGGGACTGCGCCTGCAGGAACCCCTGGTGCCCCACCCCTG
GCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCTGCCATTGGAGC
CCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTC
TTGCGGAGGGTCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGC
AGCACCAGCTCAGGGCTGGAGGTGGTGGCTCCTGAGGGTACCTCAGCCCC
AGGTGGTGGCCCGGGAACCCTGGATGACAGTGCCACCATTTGCCGTGTCT
GCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGAGTTTTGTTTC
CACCTGGACTGTCACCTGCCGGCCCTGCAGGATGTACCAGGGGAGGAGTG
GAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGAGGAGGATGGCA
GCCTCAGCCTGGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCA
CCAGCCAACCAGCGGAAATGTGAGCGTGTACTGCTGGCCCTATTCTGTCA
CGAACCCTGCCGCCCCCTGCATCAGCTGGCTACCGACTCCACCTTCTCCC
TGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCGTGCCCGCCTC
CAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGA
TGTGGGCCGCATGTTCAAGCAATTCAACAAGTTAACTGAGGACAAGGCAG
ACGTGCAGTCCATCATCGGCCTGCAGCGCTTCTTCGAGACGCGCATGAAC
GAGGCCTTCGGTGACACCAAGTTCTCTGCTGTGCTGGTGGAGCCCCCGCC
GATGAGCCTGCCTGGTGCTGGCCTGAGTTCCCAGGAGCTGTCTGGTGGCC
CTGGTGATGGCCCCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQS
ANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQRNAFIQS
LKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGRSMEKRR
WKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPGSSRKGTIDAAA
MAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASS
PAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAA
NSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDAN
QCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKS
RDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVR
NQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAIL
QIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFAS
WALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTK
SAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYG
FGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTAD
SQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGM
AIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTS
SGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLD
CHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPAN
QRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEK
LSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAF
GDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter TAP for mammalian expression.

Estimated MW of expressed protein : 110839 Daltons

100042 R 1000 1001 1047 19007

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