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BAP1

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Plasmid
pGEX6P-1-BAP1
Parent Plasmid
pGEX6P1
Expressed
GST-BAP1
DU Number
DU12809
Genbank
AAH01596.1
Tag
GST
Sequence of Insert
GGATCCATGAATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTT
CACCCTGCTCGTGGAAGATTTCGGTGTCAAGGGGGTGCAAGTGGAGGAGA
TCTACGACCTTCAGAGCAAATGTCAGGGCCCTGTATATGGATTTATCTTC
CTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAGGTCTCTACCTT
GGTGGATGATACGTCCGTGATTGATGATGATATTGTGAATAACATGTTCT
TTGCCCACCAGCTGATACCCAACTCTTGTGCAACTCATGCCTTGCTGAGC
GTGCTCCTGAACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCAT
GAAGGACTTCACCAAGGGTTTTAGCCCTGAGAGCAAAGGATATGCGATTG
GCAATGCCCCGGAGTTGGCCAAGGCCCATAATAGCCATGCCAGGCCCGAG
CCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGTGCGGACCAT
GGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTTTG
AGCTGGATGGGCTGAAGGTCTACCCCATTGACCATGGGCCCTGGGGGGAG
GACGAGGAGTGGACAGACAAGGCCCGGCGGGTCATCATGGAGCGTATCGG
CCTCGCCACTGCAGGGGAGCCCTACCACGACATCCGCTTCAACCTGATGG
CAGTGGTGCCCGACCGCAGGATCAAGTATGAGGCCAGGCTGCATGTGCTG
AAGGTGAACCGTCAGACAGTACTAGAGGCTCTGCAGCAGCTGATAAGAGT
AACACAGCCAGAGCTGATTCAGACCCACAAGTCTCAAGAGTCACAGCTGC
CTGAGGAGTCCAAGTCAGCCAGCAACAAGTCCCCGCTGGTGCTGGAAGCA
AACAGGGCCCCTGCAGCCTCTGAGGGCAACCACACAGATGGTGCAGAGGA
GGCGGCTGGTTCATGCGCACAAGCCCCATCCCACAGCCCTCCCAACAAAC
CCAAGCTAGTGGTGAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCC
AACCCCACTCCCATTGTCCAGCGGCTGCCGGCCTTTCTAGACAATCACAA
TTATGCCAAGTCCCCCATGCAGGAGGAAGAAGACCTGGCGGCAGGTGTGG
GCCGCAGCCGAGTTCCAGTCCGCCCACCCCAGCAGTACTCAGATGATGAG
GATGACTATGAGGATGACGAGGAGGATGACGTGCAGAACACCAACTCTGC
CCTTAGGTATAAGGGGAAGGGAACAGGGAAGCCAGGGGCATTGAGCGGTT
CTGCTGATGGGCAACTGTCAGTGCTGCAGCCCAACACCATCAACGTCTTG
GCTGAGAAGCTCAAAGAGTCCCAGAAGGACCTCTCAATTCCTCTGTCCAT
CAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGTGCCCACACACT
CGCAGCCCTCACCCACCCCCAGCAATGAGAGTACAGACACGGCCTCTGAG
ATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAGCCAA
CCCGACGCGGCCCTCCAGCCCTGTCACCTCCCACATCTCCAAGGTGCTTT
TTGGAGAGGATGACAGCCTGCTGCGTGTTGACTGCATACGCTACAACCGT
GCTGTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGC
TGAGGATGGGGTGCTGAGTCCCCTGGCGCTGACAGAGGGTGGGAAGGGTT
CCTCGCCCTCCATCAGACCAATCCAAGGCAGCCAGGGGTCCAGCAGCCCA
GTGGAGAAGGAGGTCGTGGAAGCCACGGACAGCAGAGAGAAGACGGGGAT
GGTGAGGCCTGGCGAGCCCTTGAGTGGGGAGAAATACTCACCCAAGGAGC
TGCTGGCACTGCTGAAGTGTGTGGAGGCTGAGATTGCAAACTATGAGGCG
TGCCTCAAGGAGGAGGTAGAGAAGAGGAAGAAGTTCAAGATTGATGACCA
GAGAAGGACCCACAACTACGATGAGTTCATCTGCACCTTTATCTCCATGC
TGGCTCAGGAAGGCATGCTGGCCAACCTAGTGGAGCAGAACATCTCCGTG
CGGCGGCGCCAAGGGGTCAGCATCGGCCGGCTCCACAAGCAGCGGAAGCC
TGACCGGCGGAAACGCTCTCGCCCCTACAAGGCCAAGCGCCAGTGAGCGG
CCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNKGWLELESDPGLFTLLV
EDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDT
SVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLSRMKDFT
KGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFH
FVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATA
GEPYHDIRFNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLIRVTQPE
LIQTHKSQESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGAEEAAGS
CAQAPSHSPPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDNHNYAKS
PMQEEEDLAAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTNSALRYK
GKGTGKPGALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPLSIKTSS
GAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRP
SSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGV
LSPLALTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPG
EPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTH
NYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRK
RSRPYKAKRQ
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter GST for bacterial expression.

Estimated MW of expressed protein : 107182 Daltons
100042 R 1000 1001 1047 19007
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