Pellino3

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Plasmid
pEBG6P-Pellino3
Parent Plasmid
pEBG6P
Expressed
GST-Pellino3
DU Number
DU12540
Genbank
AAH21256.1
Tag
GST
Sequence of Insert
GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTC
AGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCG
GTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATC
GTCCTGGGATGCTGTGAGGAAGGAGGTGAGGAAACCGAGGCTCAGAGAGG
GGAAGTGACTGGCCCAAGGGCACACAGCTGCTACAATGGTTGTCTGGCAA
GTGGGGACAAGGGCCGCCGGCGAAGCCGCCTGGCACTGAGCCGCCGGTCG
CACGCCAACGGGGTGAAGCCAGACGTCATGCACCACATCTCCACGCCGCT
CGTCTCCAAGGCACTGAGTAACCGTGGTCAGCACAGCATCTCGTATACAC
TGTCCCGGAGCCACTCGGTCATAGTGGAGTATACACATGATAGCGACACA
GACATGTTCCAGATTGGCCGCTCCACAGAGAACATGATTGACTTCGTGGT
AACAGACACGTCCCCTGGAGGAGGGGCTGCCGAGGGCCCTTCTGCCCAGA
GCACCATCTCCCGCTATGCCTGCCGCATCCTCTGTGACCGCCGGCCACCC
TATACTGCCCGCATCTATGCCGCTGGCTTCGACGCCTCTAGCAACATCTT
CCTTGGAGAGCGAGCGGCCAAATGGCGGACCCCAGATGGCCTGATGGATG
GACTGACCACCAATGGAGTCCTGGTGATGCACCCGGCAGGCGGCTTCTCC
GAGGACTCAGCCCCGGGTGTCTGGCGGGAGATCTCGGTCTGTGGGAATGT
GTACACATTGCGGGACAGCCGCTCAGCCCAGCAGCGGGGCAAGCTGGTAG
GTGGCCCGCTCCATTCCCCACCCCTATCCTTGCCAGGCCCTCACAAGCTG
CCCATCCCCCTGCCCAAGCCAGGAGATGATCTCCAGTCCTTCTGCCCCAG
GGAGCCCCAACAGGCTCCATATCAGGGAATCCCAGGCCCAGGGGGTTCTT
AGGCGGCCGC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMVLEGNPEVGSPR
TSDLQHRGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGCCEEGGEETEAQ
RGEVTGPRAHSCYNGCLASGDKGRRRSRLALSRRSHANGVKPDVMHHIST
PLVSKALSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENMIDF
VVTDTSPGGGAAEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASSN
IFLGERAAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVCG
NVYTLRDSRSAQQRGKLVGGPLHSPPLSLPGPHKLPIPLPKPGDDLQSFC
PREPQQAPYQGIPGPGGS*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter GST for mammalian expression.

Estimated MW of expressed protein : 62755 Daltons
100042 R 1000 1001 1047 19007
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