Pellino3

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Plasmid
pGEX6P-1-Pellino3
Parent Plasmid
pGEX6P1
Expressed
GST-Pellino3
DU Number
DU12541
Genbank
AAH21256.1
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTC
AGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCG
GTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATC
GTCCTGGGATGCTGTGAGGAAGGAGGTGAGGAAACCGAGGCTCAGAGAGG
GGAAGTGACTGGCCCAAGGGCACACAGCTGCTACAATGGTTGTCTGGCAA
GTGGGGACAAGGGCCGCCGGCGAAGCCGCCTGGCACTGAGCCGCCGGTCG
CACGCCAACGGGGTGAAGCCAGACGTCATGCACCACATCTCCACGCCGCT
CGTCTCCAAGGCACTGAGTAACCGTGGTCAGCACAGCATCTCGTATACAC
TGTCCCGGAGCCACTCGGTCATAGTGGAGTATACACATGATAGCGACACA
GACATGTTCCAGATTGGCCGCTCCACAGAGAACATGATTGACTTCGTGGT
AACAGACACGTCCCCTGGAGGAGGGGCTGCCGAGGGCCCTTCTGCCCAGA
GCACCATCTCCCGCTATGCCTGCCGCATCCTCTGTGACCGCCGGCCACCC
TATACTGCCCGCATCTATGCCGCTGGCTTCGACGCCTCTAGCAACATCTT
CCTTGGAGAGCGAGCGGCCAAATGGCGGACCCCAGATGGCCTGATGGATG
GACTGACCACCAATGGAGTCCTGGTGATGCACCCGGCAGGCGGCTTCTCC
GAGGACTCAGCCCCGGGTGTCTGGCGGGAGATCTCGGTCTGTGGGAATGT
GTACACATTGCGGGACAGCCGCTCAGCCCAGCAGCGGGGCAAGCTGGTAG
GTGGCCCGCTCCATTCCCCACCCCTATCCTTGCCAGGCCCTCACAAGCTG
CCCATCCCCCTGCCCAAGCCAGGAGATGATCTCCAGTCCTTCTGCCCCAG
GGAGCCCCAACAGGCTCCATATCAGGGAATCCCAGGCCCAGGGGGTTCTT
AGGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVLEGNPEVGSPRTSDLQH
RGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGCCEEGGEETEAQRGEVTG
PRAHSCYNGCLASGDKGRRRSRLALSRRSHANGVKPDVMHHISTPLVSKA
LSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENMIDFVVTDTS
PGGGAAEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASSNIFLGER
AAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVCGNVYTLR
DSRSAQQRGKLVGGPLHSPPLSLPGPHKLPIPLPKPGDDLQSFCPREPQQ
APYQGIPGPGGS
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter GST for bacterial expression.

Estimated MW of expressed proteins : 62161 Daltons
100042 R 1000 1001 1047 19007
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