USP17 | Ubiquitylation Resource

Plasmid

pEBG6P-USP17

Parent Plasmid

pEBG6P

Expressed

GST-USP17

DU Number

DU12913

Genbank

NP_001099132

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGAGGACGACTCACTCTACTTGGGAGGTGAGTGGCAGTTCAA
CCACTTTCCAAAACTCACATCTTCTCGGCCCGATGCAGCTTTTGCTGAAA
TCCAGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATGTGAGACCCGT
GTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAG
GGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGCTGCGGTGGGGGCTGGGC
TCCAGAATATGGGAAATACCTGCTACGTGAACGCTTCCTTGGAGTGGCTG
ACATACACACCGCCCCTTGCCAACTACATGCTGTCCCGGGAGCACTCTCA
AACGTGTCATCGTCACAAGGGCTGCATGCTCTGTACTATGCAAGCTCACA
TCACACGGGCCCTCCACAATCCTGGCCACGTCATCCAGCCCTCACAGGCA
TTGGCTGCTGGCTTCCATAGAGGCAAGCAGGAAGATGCTCATGAATTTCT
CATGTTCACTGTGGATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAGC
AGGTAGATCATCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGA
GGCTACTGGAGATCTCAAATCAAGTGTCTCCACTGCCACGGCATTTCAGA
CACTTTTGACCCTTACCTGGACATCGCCCTGGATATCCAGGCAGCTCAGA
GTGTCCAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGAACTCAATGGA
GAGAATGCCTATCATTGTGGTGTTTGTCTCCAGAGGGCGCCGGCCTCCAA
GACGTTAACTTTACACACCTCTGCCAAGGTCCTCATCCTTGTATTGAAGA
GATTCTCCGATGTCACAGGCAACAAGATTGCCAAGAATGTGCAATATCCT
GAGTGCCTTGACATGCAGCCATACATGTCTCAGCAGAACACAGGACCTCT
CGTCTATGTCCTCTATGCTGTGCTGGTCCACGCTGGGTGGAGTTGTCACA
ACGGACATTACTTCTCTTATGTCAAAGCTCAAGAAGGCCAGTGGTATAAA
ATGGATGATGCCGAGGTCACCGCCTCTAGCATCACTTCTGTCCTGAGTCA
ACAGGCCTACGTCCTCTTTTACATCCAGAAGAGTGAATGGGAAAGACACA
GTGAGAGTGTGTCAAGAGGCAGGGAACCAAGAGCCCTTGGCGCAGAAGAC
ACAGACAGGCGAGCAACGCAAGGAGAGCTCAAGAGAGACCACCCCTGCCT
CCAGGCCCCCGAGTTGGACGAGCACTTGGTGGAAAGAGCCACTCAGGAAA
GCACCTTAGACCACTGGAAATTCCTTCAAGAGCAAAACAAAACGAAGCCT
GAGTTCAACGTCAGAAAAGTCGAAGGTACCCTGCCTCCCGACGTACTTGT
GATTCATCAATCAAAATACAAGTGTGGGATGAAGAACCATCATCCTGAAC
AGCAAAGCTCCCTGCTAAACCTCTCTTCGACGACACCGACACATCAGGAG
TCCATGAACACTGGCACACTCGCTTCCCTGCGAGGGAGGGCCAGGAGATC
CAAAGGGAAGAACAAACACAGCAAGAGGGCTCTGCTTGTGTGCCAGTGAG
CGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEDDSLYLGGEWQ
FNHFPKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVARQLA
PREKLPLSSRRPAAVGAGLQNMGNTCYVNASLEWLTYTPPLANYMLSREH
SQTCHRHKGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHE
FLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGI
SDTFDPYLDIALDIQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPA
SKTLTLHTSAKVLILVLKRFSDVTGNKIAKNVQYPECLDMQPYMSQQNTG
PLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASSITSVL
SQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHP
CLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDV
LVIHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRAR
RSKGKNKHSKRALLVCQ*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression.

Estimated MW of expressed protein : 87165 Daltons

100042 R 1000 1001 1047 19007