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Pellino3b

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Plasmid
pEBG6P-Pellino3b
Parent Plasmid
pEBG6P
Expressed
GST-Pellino3b
DU Number
DU12156
Genbank
AAO49466.1
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTC
AGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCG
GTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATC
GTCCTGGGCTACAATGGTTGTCTGGCAAGTGGGGACAAGGGCCGCCGGCG
AAGCCGCCTGGCACTGAGCCGCCGGTCGCACGCCAACGGGGTGAAGCCAG
ACGTCATGCACCACATCTCCACGCCGCTCGTCTCCAAGGCACTGAGTAAC
CGTGGTCAGCACAGCATCTCGTATACACTGTCCCGGAGCCACTCGGTCAT
AGTGGAGTATACACATGATAGCGACACAGACATGTTCCAGATTGGCCGCT
CCACAGAGAACATGATTGACTTCGTGGTAACAGACACGTCCCCTGGAGGA
GGGGCTGCCGAGGGCCCTTCTGCCCAGAGCACCATCTCCCGCTATGCCTG
CCGCATCCTCTGTGACCGCCGGCCACCCTATACTGCCCGCATCTATGCCG
CTGGCTTCGATGCCTCTAGCAACATCTTCCTTGGAGAGCGAGCGGCCAAA
TGGCGGACCCCAGATGGCCTGATGGATGGACTGACCACCAATGGAGTCCT
GGTGATGCACCCGGCAGGCGGCTTCTCCGAGGACTCAGCCCCGGGTGTCT
GGCGGGAGATCTCGGTCTGTGGGAATGTGTACACATTGCGGGACAGCCGC
TCAGCCCAGCAGCGGGGCAAGCTGGTGGAAAACGAGTCCAACGTGCTGCA
GGACGGCTCTCTCATCGACCTGTGTGGGGCCACACTGCTGTGGCGCACAC
CGGCGGGGCTGCTGCGGGCTCCCACACTGAAGCAACTGGAGGCCCAGCGG
CAGGAGGCAAATGCAGCGCGGCCCCAGTGCCCCGTGGGCCTCAGCACTCT
GGCCTTCCCCAGCCCAGCCCGTGGCCGCACAGCGCCCGACAAACAGCAGC
CCTGGGTCTACGTCCGCTGCGGGCACGTCCATGGCTACCACGGCTGGGGC
TGCCGGCGGGAGCGGGGCCCCCAGGAGCGCGAATGTCCTCTCTGCCGCCT
TGTGGGGCCTTATGTGCCTCTATGGCTTGGCCAGGAGGCCGGCCTCTGCC
TGGACCCTGGGCCGCCTAGCCATGCCTTTGCACCTTGCGGCCACGTCTGC
TCTGAGAAGACTGCCCGCTACTGGGCCCAGACACCACTGCCCCACGGCAC
CCATGCTTTCCATGCCGCCTGCCCCTTTTGCGGGGCCTGGCTTACCGGCG
AGCATGGCTGCGTCCGCCTCATTTTCCAGGGCCCGCTGGATTAGGCGGCC
GC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMVLEGNPEVGSPR
TSDLQHRGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGYNGCLASGDKGR
RRSRLALSRRSHANGVKPDVMHHISTPLVSKALSNRGQHSISYTLSRSHS
VIVEYTHDSDTDMFQIGRSTENMIDFVVTDTSPGGGAAEGPSAQSTISRY
ACRILCDRRPPYTARIYAAGFDASSNIFLGERAAKWRTPDGLMDGLTTNG
VLVMHPAGGFSEDSAPGVWREISVCGNVYTLRDSRSAQQRGKLVENESNV
LQDGSLIDLCGATLLWRTPAGLLRAPTLKQLEAQRQEANAARPQCPVGLS
TLAFPSPARGRTAPDKQQPWVYVRCGHVHGYHGWGCRRERGPQERECPLC
RLVGPYVPLWLGQEAGLCLDPGPPSHAFAPCGHVCSEKTARYWAQTPLPH
GTHAFHAACPFCGAWLTGEHGCVRLIFQGPLD*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter GST for mammalian expression.

Estimated MW of expressed protein : 75652 Daltons
100042 R 1000 1001 1047 19007
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