Pellino3b | Ubiquitylation Resource

Plasmid

pGEX6P-1-Pellino3b

Parent Plasmid

pGEX6P1

Expressed

GST-Pellino3b

DU Number

DU12158

Genbank

AAO49466.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTC
AGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCG
GTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATC
GTCCTGGGCTACAATGGTTGTCTGGCAAGTGGGGACAAGGGCCGCCGGCG
AAGCCGCCTGGCACTGAGCCGCCGGTCGCACGCCAACGGGGTGAAGCCAG
ACGTCATGCACCACATCTCCACGCCGCTCGTCTCCAAGGCACTGAGTAAC
CGTGGTCAGCACAGCATCTCGTATACACTGTCCCGGAGCCACTCGGTCAT
AGTGGAGTATACACATGATAGCGACACAGACATGTTCCAGATTGGCCGCT
CCACAGAGAACATGATTGACTTCGTGGTAACAGACACGTCCCCTGGAGGA
GGGGCTGCCGAGGGCCCTTCTGCCCAGAGCACCATCTCCCGCTATGCCTG
CCGCATCCTCTGTGACCGCCGGCCACCCTATACTGCCCGCATCTATGCCG
CTGGCTTCGATGCCTCTAGCAACATCTTCCTTGGAGAGCGAGCGGCCAAA
TGGCGGACCCCAGATGGCCTGATGGATGGACTGACCACCAATGGAGTCCT
GGTGATGCACCCGGCAGGCGGCTTCTCCGAGGACTCAGCCCCGGGTGTCT
GGCGGGAGATCTCGGTCTGTGGGAATGTGTACACATTGCGGGACAGCCGC
TCAGCCCAGCAGCGGGGCAAGCTGGTGGAAAACGAGTCCAACGTGCTGCA
GGACGGCTCTCTCATCGACCTGTGTGGGGCCACACTGCTGTGGCGCACAC
CGGCGGGGCTGCTGCGGGCTCCCACACTGAAGCAACTGGAGGCCCAGCGG
CAGGAGGCAAATGCAGCGCGGCCCCAGTGCCCCGTGGGCCTCAGCACTCT
GGCCTTCCCCAGCCCAGCCCGTGGCCGCACAGCGCCCGACAAACAGCAGC
CCTGGGTCTACGTCCGCTGCGGGCACGTCCATGGCTACCACGGCTGGGGC
TGCCGGCGGGAGCGGGGCCCCCAGGAGCGCGAATGTCCTCTCTGCCGCCT
TGTGGGGCCTTATGTGCCTCTATGGCTTGGCCAGGAGGCCGGCCTCTGCC
TGGACCCTGGGCCGCCTAGCCATGCCTTTGCACCTTGCGGCCACGTCTGC
TCTGAGAAGACTGCCCGCTACTGGGCCCAGACACCACTGCCCCACGGCAC
CCATGCTTTCCATGCCGCCTGCCCCTTTTGCGGGGCCTGGCTTACCGGCG
AGCATGGCTGCGTCCGCCTCATTTTCCAGGGCCCGCTGGATTAGGCGGCC
GC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVLEGNPEVGSPRTSDLQH
RGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGYNGCLASGDKGRRRSRLA
LSRRSHANGVKPDVMHHISTPLVSKALSNRGQHSISYTLSRSHSVIVEYT
HDSDTDMFQIGRSTENMIDFVVTDTSPGGGAAEGPSAQSTISRYACRILC
DRRPPYTARIYAAGFDASSNIFLGERAAKWRTPDGLMDGLTTNGVLVMHP
AGGFSEDSAPGVWREISVCGNVYTLRDSRSAQQRGKLVENESNVLQDGSL
IDLCGATLLWRTPAGLLRAPTLKQLEAQRQEANAARPQCPVGLSTLAFPS
PARGRTAPDKQQPWVYVRCGHVHGYHGWGCRRERGPQERECPLCRLVGPY
VPLWLGQEAGLCLDPGPPSHAFAPCGHVCSEKTARYWAQTPLPHGTHAFH
AACPFCGAWLTGEHGCVRLIFQGPLD*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter GST for bacterial expression.

Estimated MW of expressed protein : 75058 Daltons

100042 R 1000 1001 1047 19007