Pellino2 | Ubiquitylation Resource

Plasmid

pGEX6P-1-Pellino2 new

Parent Plasmid

pGEX6P1

Expressed

GST Pellino2

DU Number

DU15004

Genbank

AAH09476.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGA
GCCAGTGAAATACGGGGAGCTGGTGGTGCTCGGGTACAATGGTGCTTTAC
CCAATGGAGATAGAGGACGGAGGAAAAGTAGATTTGCCCTCTACAAGCGG
CCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCATGTGATATCCACGCC
CCAGGCATCCAAGGCTATCAGCTGCAAAGGTCAACACAGTATATCCTACA
CTTTGTCAAGGAATCAGACTGTGGTGGTGGAGTACACACATGATAAGGAT
ACGGATATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGT
TGTCACAGACACGATTTCTGGCAGCCAGAACACGGACGAAGCCCAGATCA
CACAGAGCACCATATCCAGGTTCGCCTGCAGGATCGTGTGCGACAGGAAT
GAACCTTACACAGCACGGATATTCGCCGCCGGATTTGACTCTTCCAAAAA
CATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGCCACA
TGGATGGGCTCACTACTAATGGCGTCCTGGTGATGCATCCACGAGGGGGC
TTCACCGAGGAGTCCCAGCCCGGGGTCTGGCGCGAGATCTCTGTCTGTGG
AGATGTGTACACCTTGCGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGC
TGGTGGAAAGTGAGACCAACGTCCTGCAGGACGGCTCCCTCATTGACCTG
TGTGGGGCCACTCTCCTCTGGAGAACAGCAGATGGGCTTTTTCATACTCC
AACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCCCGGC
CTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGG
AAAGAGGTGGTGGAGGAGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCA
CGTGCACGGGTACCACAACTGGGGCCATCGGAGTGACACGGAGGCCAACG
AGAGGGAGTGTCCCATGTGCAGGACTGTGGGCCCCTATGTGCCTCTCTGG
CTTGGCTGTGAGGCAGGATTTTATGTAGACGCAGGACCGCCAACTCATGC
TTTCACTCCCTGTGGACACGTGTGCTCGGAGAAGTCTGCAAAATACTGGT
CTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTGCCCT
TTCTGTGCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTT
CCAAGGTCCAATTGACTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFSPGQEEHCAPNKEPVKY
GELVVLGYNGALPNGDRGRRKSRFALYKRPKANGVKPSTVHVISTPQASK
AISCKGQHSISYTLSRNQTVVVEYTHDKDTDMFQVGRSTESPIDFVVTDT
ISGSQNTDEAQITQSTISRFACRIVCDRNEPYTARIFAAGFDSSKNIFLG
EKAAKWKNPDGHMDGLTTNGVLVMHPRGGFTEESQPGVWREISVCGDVYT
LRETRSAQQRGKLVESETNVLQDGSLIDLCGATLLWRTADGLFHTPTQKH
IEALRQEINAARPQCPVGLNTLAFPSINRKEVVEEKQPWAYLSCGHVHGY
HNWGHRSDTEANERECPMCRTVGPYVPLWLGCEAGFYVDAGPPTHAFTPC
GHVCSEKSAKYWSQIPLPHGTHAFHAACPFCATQLVGEQNCIKLIFQGPI
D*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter GST for bacterial expression.

Estimated MW of expressed protein : 73257 Daltons

100042 R 1000 1001 1047 19007