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Pellino1

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Plasmid
pFBHTb Pellino1
Parent Plasmid
pFastBac HTb
Expressed
6His-Pellino1
DU Number
DU9000
Genbank
Q96FA3
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGTTTTCTCCTGATCAAGAAAATCATCCATTTAAAGCACCAGT
AAAATATGGTGAACTCATTGTCTTAGGGTATAATGGGTCTCTCCCAAATG
GCGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACCTAAG
GCAAATGGGGTGAAGCCCAGCACTGTGCATATTGCTTGTACTCCTCAGGC
TGCAAAGGCAATAAGCAACAAAGACCAGCATAGCATATCATATACTTTGT
CTCGGGCCCAGACTGTGGTGGTTGAATATACTCATGACAGCAACACAGAT
ATGTTTCAGATTGGCCGGTCGACTGAAAGCCCCATTGATTTTGTAGTAAC
TGACACAGTTCCTGGAAGTCAAAGTAATTCTGATACACAGTCAGTACAAA
GCACTATATCAAGATTTGCCTGCAGAATCATATGTGAACGGAATCCTCCC
TTTACAGCACGGATTTATGCTGCAGGATTTGACTCATCAAAAAACATCTT
TCTTGGGGAGAAGGCTGCCAAATGGAAGACATCAGATGGACAGATGGATG
GCTTGACCACTAATGGTGTTCTTGTGATGCATCCACGCAATGGGTTCACA
GAAGACTCCAAGCCTGGAATATGGAGAGAAATATCGGTGTGTGGAAATGT
ATTTAGCCTACGTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGG
AAATTGAAACCAATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGT
GCAACATTGTTATGGCGTACTGCAGAAGGCCTTTCCCACACTCCTACCGT
GAAGCATTTAGAAGCTTTAAGACAGGAAATCAATGCAGCACGACCTCAGT
GCCCTGTAGGGTTCAACACACTAGCATTTCCTAGTATGAAGAGGAAAGAC
GTTGTAGATGAAAAACAACCATGGGTATATCTAAACTGCGGCCATGTACA
TGGCTATCATAACTGGGGAAACAAAGAAGAACGTGATGGAAAAGATCGTG
AATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGA
TGTGAAGCTGGATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAG
CCCGTGTGGGCATGTGTGTTCAGAAAAGACAACTGCCTATTGGTCCCAGA
TCCCACTTCCTCATGGTACTCATACTTTTCATGCAGCCTGTCCCTTTTGT
GCACATCAGTTGGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGG
ACCTCTAGACTAAGCGGCCGC
Amino Acid Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSMFSPDQENHPFKAPVKYGELIV
LGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAISNK
DQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQ
SNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEKAAK
WKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCGNVFSLRETR
SAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLEALR
QEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGHVHGYHNWGN
KEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHAFSPCGHVCS
EKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
N ter 6His for baculovirus expression.

Estimated MW of expressed protein : 49716 Daltons
100042 R 1000 1001 1047 19007
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