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Synonyms
PHA2D
Plasmid
pBABED P U6 KLHL3 KO sense B
Parent Plasmid
pBABED P U6
Expressed
KLHL3 KO sense guide B
DU Number
DU48899
Genbank
ENSG00000146021
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
Comments
CRISPR guide plasmid. Targets sense strand in exon 5, designed to KO KLHL3 when co-transfected with antisense guide 48898. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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