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Plasmid
pSc-B-6HIS(TAG3)-UBCH7 C17S S91A C137S (Killed internal Nco1)
Parent Plasmid
pSC-B
DU Number
DU24644
Genbank
P68036
Species
Human
Sequence of Insert
View Sequence
CCATGGGCTAGAGCCATCATCATCATCATCACAGCAGCGGCCTGGAAGTTCTGTTCCAGGGGCCCGGATCTATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATCTGGGATGAAAAACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAGGGGCAGGTCTGTCTGCCAGTAATTGCTGCCGAAAACTGGAAGCCAGCAACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTCTAAGAATGCTGAAGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAACTCGAG
Amino Acid Sequence
View Sequence
MG*SHHHHHHSSGLEVLFQGPGSMAASRRLMKELEEIRKSGMKNFRNIQV
DEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHP
NIDEKGQVCLPVIAAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAE
EYSKDRKKFSKNAEEFTKKYGEKRPVD*
DEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHP
NIDEKGQVCLPVIAAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAE
EYSKDRKKFSKNAEEFTKKYGEKRPVD*
Vector Type
Other
Antibiotic
Amp; Kan
Comments
native internal Nco1 site between tag and M1 of ORF killed
100042 R 1000 1001 1047 19007
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