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Synonyms
HP43.8KD
Plasmid
pcDNA5 FRT/TO BirA R118G-USP38-HA
Parent Plasmid
pcDNA5 FRT/TO BirA R118G MCS HA
Expressed
BirA R118G-USP38-HA
DU Number
DU49897
Genbank
NM_032557.5 GI:195539360 with silent changes t999c and g2589a
Tag
HA
BirA
Species
Human
Sequence of Insert
View Sequence
GAATTCATGGACAAGATCCTGGAGGGCCTTGTGAGTTCCTCGCATCCCCTGCCCCTCAAGCGGGTGATTGTGCGGAAGGTGGTGGAATCGGCGGAGCACTGGCTAGACGAGGCGCAGTGCGAGGCCATGTTTGACCTGACGACCCGGCTCATCCTGGAGGGCCAGGACCCTTTCCAGCGGCAGGTGGGGCACCAGGTGCTGGAGGCCTACGCACGATACCACCGGCCAGAGTTCGAGTCCTTCTTCAACAAGACCTTCGTGTTGGGCCTCCTTCATCAGGGCTACCACTCTCTGGACAGGAAGGATGTAGCCATCCTGGACTACATTCACAACGGCCTGAAGCTGATTATGAGCTGTCCGTCGGTGCTGGATCTCTTTAGCCTCCTGCAGGTAGAGGTGTTACGGATGGTGTGTGAGAGGCCGGAGCCGCAGCTCTGTGCCCGACTGAGCGACCTTCTGACCGACTTTGTGCAATGCATCCCCAAGGGGAAATTGTCCATCACGTTCTGTCAACAGCTGGTTCGAACGATAGGCCATTTCCAGTGCGTGTCCACCCAGGAAAGAGAGCTGCGGGAATATGTCTCCCAGGTGACAAAAGTGAGTAACTTGCTGCAGAACATCTGGAAGGCCGAGCCTGCCACACTACTGCCTTCCCTGCAAGAAGTTTTTGCAAGCATCTCTTCCACAGATGCATCATTTGAACCTTCTGTAGCATTGGCAAGCCTTGTGCAGCATATTCCTCTTCAGATGATTACAGTTCTCATCAGGAGCCTTACTACGGATCCAAATGTAAAAGATGCAAGTATGACCCAAGCCCTTTGCAGAATGATTGACTGGCTATCCTGGCCATTGGCTCAGCATGTGGATACATGGGTAATTGCACTCCTGAAAGGACTGGCAGCTGTCCAGAAGTTTACTATTTTGATAGATGTTACTTTGCTGAAAATAGAACTGGTTTTTAATCGACTTTGGTTTCCTCTTGTGAGACCTGGTGCTCTTGCAGTCCTTTCTCACATGCTGCTTAGCTTTCAGCATTCTCCAGAGGCGTTCCATTTGATTGTTCCTCATGTGGTTAATTTGGTTCATTCTTTCAAAAATGATGGTCTGCCTTCAAGTACAGCCTTCTTAGTACAATTAACAGAATTGATACACTGTATGATGTATCATTATTCTGGATTTCCAGATCTCTATGAACCTATTCTGGAGGCAATAAAGGATTTTCCTAAGCCCAGTGAAGAGAAGATTAAGTTAATTCTCAATCAAAGTGCCTGGACTTCTCAATCCAATTCTTTGGCGTCTTGCTTGTCTAGACTTTCTGGAAAATCTGAAACTGGGAAAACTGGTCTTATTAACCTAGGAAATACATGTTATATGAACAGTGTTATACAAGCCTTGTTTATGGCCACAGATTTCAGGAGACAAGTATTATCTTTAAATCTAAATGGGTGCAATTCATTAATGAAAAAATTACAGCATCTTTTTGCCTTTCTGGCCCATACACAGAGGGAAGCATACGCACCTCGGATATTCTTTGAGGCTTCCAGACCTCCATGGTTTACTCCCAGATCACAGCAAGACTGTTCTGAATACCTCAGATTTCTCCTTGACAGGCTCCATGAAGAAGAAAAGATCTTGAAAGTTCAGGCCTCACACAAGCCTTCTGAAATTCTGGAATGCAGTGAAACTTCTTTACAGGAAGTAGCTAGTAAAGCAGCAGTACTAACAGAGACCCCTCGTACAAGTGACGGTGAGAAGACTTTAATAGAAAAAATGTTTGGAGGAAAACTACGAACTCACATACGTTGTTTGAACTGCAGGAGTACCTCACAAAAAGTGGAAGCCTTTACAGATCTTTCGCTTGCCTTTTGTCCTTCCTCTTCTTTGGAAAACATGTCTGTCCAAGATCCAGCATCATCACCCAGTATACAAGATGGTGGTCTAATGCAAGCCTCTGTACCCGGTCCTTCAGAAGAACCAGTAGTTTATAATCCAACAACAGCTGCCTTCATCTGTGACTCACTTGTGAATGAAAAAACCATAGGCAGTCCTCCTAATGAGTTTTACTGTTCTGAAAACACTTCTGTCCCTAACGAATCTAACAAGATTCTTGTTAATAAAGATGTACCTCAGAAACCAGGAGGTGAAACCACACCTTCAGTAACTGACTTACTAAATTATTTTTTGGCTCCAGAGATTCTTACTGGTGATAACCAATATTATTGTGAAAACTGTGCCTCTCTGCAAAATGCTGAGAAAACTATGCAAATCACGGAGGAACCTGAATACCTTATTCTTACTCTCCTGAGATTTTCATATGATCAGAAGTATCATGTGAGAAGGAAAATTTTAGACAATGTATCACTGCCACTGGTTTTGGAGTTGCCAGTTAAAAGAATTACTTCTTTCTCTTCATTGTCAGAAAGTTGGTCTGTAGATGTTGACTTCACTGATCTTAGTGAGAACCTTGCTAAAAAATTAAAGCCTTCAGGGACTGATGAAGCTTCCTGCACAAAATTGGTGCCCTATCTATTAAGTTCCGTTGTGGTTCACTCTGGTATATCCTCTGAAAGTGGGCATTACTATTCTTATGCCAGAAATATCACAAGTACAGACTCTTCATATCAGATGTACCACCAGTCTGAGGCTCTGGCATTAGCATCCTCCCAGAGTCATTTACTAGGGAGAGATAGTCCCAGTGCAGTTTTTGAACAGGATTTGGAAAATAAGGAAATGTCAAAAGAATGGTTTTTATTTAATGACAGTAGAGTGACATTTACTTCATTTCAGTCAGTCCAGAAAATTACGAGCAGGTTTCCAAAGGACACAGCTTATGTGCTTTTGTATAAAAAACAGCATAGTACTAATGGTTTAAGTGGTAATAACCCAACCAGTGGACTCTGGATAAATGGAGACCCACCTCTACAGAAAGAACTTATGGATGCTATAACAAAAGACAATAAACTATATTTACAGGAACAAGAGTTGAATGCTCGAGCCCGGGCCCTCCAAGCTGCATCTGCTTCATGTTCATTTCGGCCCAATGGATTTGATGACAACGACCCACCAGGAAGCTGTGGACCAACTGGTGGAGGGGGTGGAGGAGGATTTAATACAGTTGGCAGACTCGTATTTGCGGCCGC
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFMDKILEGLVSSSHPLPLKRVIVR
KVVESAEHWLDEAQCEAMFDLTTRLILEGQDPFQRQVGHQVLEAYARYHR
PEFESFFNKTFVLGLLHQGYHSLDRKDVAILDYIHNGLKLIMSCPSVLDL
FSLLQVEVLRMVCERPEPQLCARLSDLLTDFVQCIPKGKLSITFCQQLVR
TIGHFQCVSTQERELREYVSQVTKVSNLLQNIWKAEPATLLPSLQEVFAS
ISSTDASFEPSVALASLVQHIPLQMITVLIRSLTTDPNVKDASMTQALCR
MIDWLSWPLAQHVDTWVIALLKGLAAVQKFTILIDVTLLKIELVFNRLWF
PLVRPGALAVLSHMLLSFQHSPEAFHLIVPHVVNLVHSFKNDGLPSSTAF
LVQLTELIHCMMYHYSGFPDLYEPILEAIKDFPKPSEEKIKLILNQSAWT
SQSNSLASCLSRLSGKSETGKTGLINLGNTCYMNSVIQALFMATDFRRQV
LSLNLNGCNSLMKKLQHLFAFLAHTQREAYAPRIFFEASRPPWFTPRSQQ
DCSEYLRFLLDRLHEEEKILKVQASHKPSEILECSETSLQEVASKAAVLT
ETPRTSDGEKTLIEKMFGGKLRTHIRCLNCRSTSQKVEAFTDLSLAFCPS
SSLENMSVQDPASSPSIQDGGLMQASVPGPSEEPVVYNPTTAAFICDSLV
NEKTIGSPPNEFYCSENTSVPNESNKILVNKDVPQKPGGETTPSVTDLLN
YFLAPEILTGDNQYYCENCASLQNAEKTMQITEEPEYLILTLLRFSYDQK
YHVRRKILDNVSLPLVLELPVKRITSFSSLSESWSVDVDFTDLSENLAKK
LKPSGTDEASCTKLVPYLLSSVVVHSGISSESGHYYSYARNITSTDSSYQ
MYHQSEALALASSQSHLLGRDSPSAVFEQDLENKEMSKEWFLFNDSRVTF
TSFQSVQKITSRFPKDTAYVLLYKKQHSTNGLSGNNPTSGLWINGDPPLQ
KELMDAITKDNKLYLQEQELNARARALQAASASCSFRPNGFDDNDPPGSC
GPTGGGGGGGFNTVGRLVFAAAYPYDVPDYA*
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFMDKILEGLVSSSHPLPLKRVIVR
KVVESAEHWLDEAQCEAMFDLTTRLILEGQDPFQRQVGHQVLEAYARYHR
PEFESFFNKTFVLGLLHQGYHSLDRKDVAILDYIHNGLKLIMSCPSVLDL
FSLLQVEVLRMVCERPEPQLCARLSDLLTDFVQCIPKGKLSITFCQQLVR
TIGHFQCVSTQERELREYVSQVTKVSNLLQNIWKAEPATLLPSLQEVFAS
ISSTDASFEPSVALASLVQHIPLQMITVLIRSLTTDPNVKDASMTQALCR
MIDWLSWPLAQHVDTWVIALLKGLAAVQKFTILIDVTLLKIELVFNRLWF
PLVRPGALAVLSHMLLSFQHSPEAFHLIVPHVVNLVHSFKNDGLPSSTAF
LVQLTELIHCMMYHYSGFPDLYEPILEAIKDFPKPSEEKIKLILNQSAWT
SQSNSLASCLSRLSGKSETGKTGLINLGNTCYMNSVIQALFMATDFRRQV
LSLNLNGCNSLMKKLQHLFAFLAHTQREAYAPRIFFEASRPPWFTPRSQQ
DCSEYLRFLLDRLHEEEKILKVQASHKPSEILECSETSLQEVASKAAVLT
ETPRTSDGEKTLIEKMFGGKLRTHIRCLNCRSTSQKVEAFTDLSLAFCPS
SSLENMSVQDPASSPSIQDGGLMQASVPGPSEEPVVYNPTTAAFICDSLV
NEKTIGSPPNEFYCSENTSVPNESNKILVNKDVPQKPGGETTPSVTDLLN
YFLAPEILTGDNQYYCENCASLQNAEKTMQITEEPEYLILTLLRFSYDQK
YHVRRKILDNVSLPLVLELPVKRITSFSSLSESWSVDVDFTDLSENLAKK
LKPSGTDEASCTKLVPYLLSSVVVHSGISSESGHYYSYARNITSTDSSYQ
MYHQSEALALASSQSHLLGRDSPSAVFEQDLENKEMSKEWFLFNDSRVTF
TSFQSVQKITSRFPKDTAYVLLYKKQHSTNGLSGNNPTSGLWINGDPPLQ
KELMDAITKDNKLYLQEQELNARARALQAASASCSFRPNGFDDNDPPGSC
GPTGGGGGGGFNTVGRLVFAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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