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Synonyms
E2-EPF, E2EPF, EPF5
Plasmid
pU6 TRIM49 Nter gRNA 2
Parent Plasmid
pU6 gRNA
Expressed
UBE2S Nter gRNA 2
DU Number
DU48401
Genbank
ENSG00000108106
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
Comments
Expresses UBE2S guide RNA, 20mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets. Targets exon 3 on reverse strand and is designed to KO UBE2S by introducing an Indel via CRISPR. To be screened for by PCR and NdeI digestion, loss of the site shall indicate the presence of an Indel and possible KO.
100042 R 1000 1001 1047 19007
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