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Plasmid
pU6 DCUN1D4 gRNA B
Parent Plasmid
pU6 gRNA BamHI
Expressed
DCUN1D4 gRNA B
DU Number
DU48397
Genbank
ENSG00000109184
Species
Human
Vector Type
Mammalian
Antibiotic
Amp
Comments
Expresses DCUN1D4 guide RNA B, 19mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets (extra G added to aid expression). Targets 1st common coding exon (4), PAM site is situated on the sense strand. To be used in conjunction with DCUN1D4 guide RNA A, a ssDNA KO donor and Cas9 nickase.
100042 R 1000 1001 1047 19007
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