Ubiquitin | Ubiquitylation Resource

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Plasmid

pET3a-Ubiquitin-SUMO3 (M1-D15)-Ubiquitin -6HIS (wild type)

Parent Plasmid

pET3a

Expressed

Ubiquitin-SUMO3 M1-D15-Ubiquitin-6His

DU Number

DU24500

Genbank

P62988

Tag

6His

Species

Human

Sequence of Insert

View Sequence

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGTCCGAGGAGAAGCCCA
AGGAGGGTGTGAAGACAGAGAATGACATGCAGATCTTCGTGAAGACCCTG
ACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAA
TGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGA
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC
TACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGG
TGGGCATCATCACCATCACCATTGATAAGGATCC

Amino Acid Sequence

View Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGMSEEKPKEGVKTENDMQIFVKTLT
GKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDY
NIQKESTLHLVLRLRGGHHHHHH**

Vector Type

Bacterial

Antibiotic

Amp

Comments

First 15 residues of SUMO3 inserted between the two ubiquitin ORFs

100042 R 1000 1001 1047 19007

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