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Plasmid
pET15b 6HIS 3C UBE2Z C261S
Parent Plasmid
pET15b 6His 3C
Expressed
His-UBE2Z C261S
DU Number
DU46572
Genbank
NM_023079.3 GI:150417998*
Tag
6His
Species
Human
Sequence of Insert
View Sequence
ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGAAGTTCTGTTCCAGGGGCCCGGATCCATGGCGGAGAGTCCGACTGAGGAGGCGGCAA
CGGCGGGCGCCGGGGCGGCGGGCCCCGGGGCGAGCAGCGTTGCTGGTGTTGTTGGCGTTAGCGGCAGCGGCGGCGGGTTCGGGCCGCCTTTCCTGCCGGA
TGTGTGGGCGGCGGCGGCGGCAGCGGGCGGGGCCGGGGGCCCGGGGAGCGGCCTGGCTCCGCTGCCCGGGCTCCCGCCCTCAGCCGCTGCCCACGGGGCC
GCGCTGCTTAGCCACTGGGACCCCACGCTCAGCTCCGACTGGGACGGCGAGCGCACCGCGCCGCAGTGTCTACTCCGGATCAAGCGGGATATCATGTCCA
TTTATAAGGAGCCTCCTCCAGGAATGTTCGTTGTACCTGATACTGTTGACATGACTAAGATTCATGCATTGATCACAGGCCCATTTGACACTCCTTATGA
AGGGGGTTTCTTCCTGTTCGTGTTTCGGTGTCCGCCCGACTATCCCATCCACCCACCTCGGGTCAAACTGATGACAACGGGCAATAACACAGTGAGGTTT
AACCCCAACTTCTACCGCAATGGGAAAGTCTGCTTGAGTATTCTAGGTACATGGACTGGACCTGCCTGGAGCCCAGCCCAGAGCATCTCCTCAGTGCTCA
TCTCTATCCAGTCCCTGATGACTGAGAACCCCTATCACAATGAGCCCGGCTTTGAACAGGAGAGACATCCAGGAGACAGCAAAAACTATAATGAATGTAT
CCGGCACGAGACCATCAGAGTTGCAGTCTGTGACATGATGGAAGGAAAGaGTCCCTGTCCTGAACCCCTACGAGGGGTGATGGAGAAGTCCTTTCTGGAG
TATTACGACTTCTATGAGGTGGCCTGCAAAGATCGCCTGCACCTTCAAGGCCAAACTATGCAGGACCCTTTTGGAGAGAAGCGGGGCCACTTTGACTACC
AGTCCCTCTTGATGCGCCTGGGACTGATACGTCAGAAAGTGCTGGAGAGGCTCCATAATGAGAATGCAGAAATGGACTCTGATAGCAGTTCATCTGGGAC
AGAGACAGACCTTCATGGGAGCCTGAGGGTTTAG
CGGCGGGCGCCGGGGCGGCGGGCCCCGGGGCGAGCAGCGTTGCTGGTGTTGTTGGCGTTAGCGGCAGCGGCGGCGGGTTCGGGCCGCCTTTCCTGCCGGA
TGTGTGGGCGGCGGCGGCGGCAGCGGGCGGGGCCGGGGGCCCGGGGAGCGGCCTGGCTCCGCTGCCCGGGCTCCCGCCCTCAGCCGCTGCCCACGGGGCC
GCGCTGCTTAGCCACTGGGACCCCACGCTCAGCTCCGACTGGGACGGCGAGCGCACCGCGCCGCAGTGTCTACTCCGGATCAAGCGGGATATCATGTCCA
TTTATAAGGAGCCTCCTCCAGGAATGTTCGTTGTACCTGATACTGTTGACATGACTAAGATTCATGCATTGATCACAGGCCCATTTGACACTCCTTATGA
AGGGGGTTTCTTCCTGTTCGTGTTTCGGTGTCCGCCCGACTATCCCATCCACCCACCTCGGGTCAAACTGATGACAACGGGCAATAACACAGTGAGGTTT
AACCCCAACTTCTACCGCAATGGGAAAGTCTGCTTGAGTATTCTAGGTACATGGACTGGACCTGCCTGGAGCCCAGCCCAGAGCATCTCCTCAGTGCTCA
TCTCTATCCAGTCCCTGATGACTGAGAACCCCTATCACAATGAGCCCGGCTTTGAACAGGAGAGACATCCAGGAGACAGCAAAAACTATAATGAATGTAT
CCGGCACGAGACCATCAGAGTTGCAGTCTGTGACATGATGGAAGGAAAGaGTCCCTGTCCTGAACCCCTACGAGGGGTGATGGAGAAGTCCTTTCTGGAG
TATTACGACTTCTATGAGGTGGCCTGCAAAGATCGCCTGCACCTTCAAGGCCAAACTATGCAGGACCCTTTTGGAGAGAAGCGGGGCCACTTTGACTACC
AGTCCCTCTTGATGCGCCTGGGACTGATACGTCAGAAAGTGCTGGAGAGGCTCCATAATGAGAATGCAGAAATGGACTCTGATAGCAGTTCATCTGGGAC
AGAGACAGACCTTCATGGGAGCCTGAGGGTTTAG
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLEVLFQGPGSMAESPTEEAATAGAGAAGPGASSVAGVVGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLPPSAAAHGA
ALLSHWDPTLSSDWDGERTAPQCLLRIKRDIMSIYKEPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRF
NPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKSPCPEPLRGVMEKSFLE
YYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV*
ALLSHWDPTLSSDWDGERTAPQCLLRIKRDIMSIYKEPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRF
NPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKSPCPEPLRGVMEKSFLE
YYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Based on DU20121, mutate C261 to S
100042 R 1000 1001 1047 19007
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