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Plasmid
pCMV5 Halo 3C1 Traf6
Parent Plasmid
pCMV5 Halo 3C1
Expressed
Halo-TRAF6
DU Number
DU46423
Genbank
NM_004620
Species
Human
Sequence of Insert
View Sequence
GGATCCAGTCTGCTAAACTGTGAAAACAGCTGTGGATTCAGCCAGTCTGA AAGTGACTGCTGTGTGGCCATGGCCAGCTCCTGTAGCGCTGTAACAAAAG ATGATAGTGTGGGTGGAACTGCCAGCACGGGGAACCTCTCCAGCTCATTT ATGGAGGAGATCCAGGGATATGATGTAGAGTTTGACCCACCCCTGGAAAG CAAGTATGAATGCCCCATCTGCTTGATGGCATTACGAGAAGCAGTGCAAA CGCCATGCGGCCATAGGTTCTGCAAAGCCTGCATCATAAAATCAATAAGG GATGCAGGTCACAAATGTCCAGTTGACAATGAAATACTGCTGGAAAATCA ACTATTTCCAGACAATTTTGCAAAACGTGAGATTCTTTCTCTGATGGTGA AATGTCCAAATGAAGGTTGTTTGCACAAGATGGAACTGAGACATCTTGAG GATCATCAAGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCAATGCCA GCGTCCCTTCCAAAAATTCCATATTAATATTCACATTCTGAAGGATTGTC CAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCATTTGAA GATAAAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCATCTGTGA ATACTGCAATACTATACTCATCAGAGAACAGATGCCTAATCATTATGATC TAGACTGCCCTACAGCCCCAATTCCATGCACATTCAGTACTTTTGGTTGC CATGAAAAGATGCAGAGGAATCACTTGGCACGCCACCTACAAGAGAACAC CCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAGTTTGAGCGTTA TACCCGACTCTGGGTATATCTCAGAGGTCCGGAATTTCCAGGAAACTATT CACCAGTTAGAGGGTCGCCTTGTAAGACAAGACCATCAAATCCGGGAGCT GACTGCTAAAATGGAAACTCAGAGTATGTATGTAAGTGAGCTCAAACGAA CCATTCGAACCCTTGAGGACAAAGTTGCTGAAATCGAAGCACAGCAGTGC AATGGAATTTATATTTGGAAGATTGGCAACTTTGGAATGCATTTGAAATG TCAAGAAGAGGAGAAACCTGTTGTGATTCATAGCCCTGGATTCTACACTG GCAAACCCGGGTACAAACTGTGCATGCGCTTGCACCTTCAGTTACCGACT GCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAATGCAAGG AGAATATGACAGCCACCTCCCTTGGCCCTTCCAGGGTACAATACGCCTTA CAATTCTTGATCAGTCTGAAGCACCTGTAAGGCAAAACCACGAAGAGATA ATGGATGCCAAACCAGAGCTGCTTGCTTTCCAGCGACCCACAATCCCACG GAACCCAAAAGGTTTTGGCTATGTAACTTTTATGCATCTGGAAGCCCTAA GACAAAGAACTTTCATTAAGGATGACACATTATTAGTGCGCTGTGAGGTC TCCACCCGCTTTGACATGGGTAGCCTTCGGAGGGAGGGTTTTCAGCCACG AAGTACTGATGCAGGGGTATAGGCGGCCGCGC
Amino Acid Sequence
View Sequence
MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRN
KKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEIS
MLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYL
NGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYL
KSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGSSLLNCENSCG
FSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFD
PPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEI
LLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMD
CPQCQRPFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLA
NVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARH
LQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDH
QIRELTAKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFG
MHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFV
HTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQR
PTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRRE
GFQPRSTDAGV*
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRN
KKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEIS
MLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYL
NGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYL
KSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGSSLLNCENSCG
FSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFD
PPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEI
LLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMD
CPQCQRPFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLA
NVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARH
LQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDH
QIRELTAKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFG
MHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFV
HTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQR
PTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRRE
GFQPRSTDAGV*
Vector Type
Mammalian
Comments
Can TRAF6 (DU15444) be cloned into DU39747 to add a Halo tag to the TRAF6. I don't mind if the HA tag is kept on TRAF6 or removed. Thanks.
100042 R 1000 1001 1047 19007
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